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Cytokeratin 14抗体

Anti-Cytokeratin 14 antibody used in IHC (Paraffin sections) (IHC-P). GTX104124
Anti-Cytokeratin 14 antibody used in Western Blot (WB). GTX104124
Anti-Cytokeratin 14 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104124
Anti-Cytokeratin 14 antibody used in Western Blot (WB). GTX104124
Anti-Cytokeratin 14 antibody used in IHC (Paraffin sections) (IHC-P). GTX104124
Anti-Cytokeratin 14 antibody used in IHC (Paraffin sections) (IHC-P). GTX104124

目录号 GTX104124

文献引用 14
2

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IHC-Fr, IHC

种属反应

 Human, Rat, Dog
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 Cytokeratin 14 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IHC Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

52 kDa. ( Note )

阳性对照

HeLa

预测种属反应

Mouse, Bovine(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.48 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 14. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1950640

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

keratin 14 , CK14 , EBS3 , EBS4 , K14 , NFJ

细胞定位

Cytoplasm , Nucleus

功能与背景

This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Cytokeratin 14 antibody used in IHC (Paraffin sections) (IHC-P). GTX104124

GTX104124 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Lung CA, using Cytokeratin 14(GTX104124) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cytokeratin 14 antibody used in Western Blot (WB). GTX104124

GTX104124 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 14 antibody (GTX104124) diluted at 1:6000.

Anti-Cytokeratin 14 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104124

GTX104124 ICC/IF Image

Cytokeratin 14 antibody detects Cytokeratin 14 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cytokeratin 14 protein stained by Cytokeratin 14 antibody (GTX104124) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Anti-Cytokeratin 14 antibody used in Western Blot (WB). GTX104124

GTX104124 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 14 antibody (GTX104124) diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cytokeratin 14 antibody used in IHC (Paraffin sections) (IHC-P). GTX104124

GTX104124 IHC-P Image

The data was published in the journal Front Oncol in 2013.PMID: 23596564

Anti-Cytokeratin 14 antibody used in IHC (Paraffin sections) (IHC-P). GTX104124

GTX104124 IHC-P Image

The data was published in the journal Nat Commun in 2019.PMID: 31455775

文献引用

Application Reference

Takayasu Moroki et al. J Toxicol Pathol 2021; 34 (2) : 161-180 Databases for technical aspects of immunohistochemistry: 2021 update.
实验应用 : IHC
种属反应 : Rat
Chongfa Tang et al. Sci Rep 2021; 11 (1) : 5848 Primary ectocervical epithelial cells display lower permissivity to Chlamydia trachomatis than HeLa cells and a globally higher pro-inflammatory profile.
实验应用 : ICC/IF
种属反应 : Human
Kun-Ling Lin et al. Int J Mol Sci 2021; 22 (17) Low-Intensity Extracorporeal Shock Wave Therapy Promotes Bladder Regeneration and Improves Overactive Bladder Induced by Ovarian Hormone Deficiency from Rat Animal Model to Human Clinical Trial.
实验应用 : WB, ICC/IF
种属反应 : Rat
Kim JY et al. Nat Commun 2019; 10 (1) : 3694 Priming mobilization of hair follicle stem cells triggers permanent loss of regeneration after alkylating chemotherapy.
实验应用 : IHC-Fr
种属反应 : Human
D'Amico S et al. Genes Dev 2018; 32 (17-18) : 1175-1187 STAT3 is a master regulator of epithelial identity and KRAS-driven tumorigenesis.
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产品评论

Cytokeratin 14 antibody 目录号 GTX104124

Rating
( Average 5 based on 2 users reviews)
实验应用
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
IHC (Frozen sections)(IHC-Fr)
( Average 5 based on 1 users reviews)
Anti-Cytokeratin 14 antibody used in IHC (Paraffin sections) (IHC-P). GTX104124
Date : Anonymous submitted on 21-Dec-2021
Score :
Application Tested : IHC-P
Sample Species : Rat
Sample : Rat Glandula sublimgualis tissue
Blocking : 0.3% H2O2 in methanol, 20min, RT and 2.5% Normal horse seru in TBST, 20min, RT
Fixation : neutral buffered formalin
Permeabilization /Antigen retrieval : TBST/citrate buffer (pH6), MW 10min
Primary Antibody : 1:200, 25°C, 1Hr
Figure Note : Duct epithelial cells and myoepithlial cells are postive. Acinar cells are negative.
Anti-Cytokeratin 14 antibody used in IHC (Frozen sections) (IHC-Fr). GTX104124
Date : Anonymous submitted on 11-Dec-2013
Score :
Application Tested : IHC-Fr
Sample Species : Rat
Sample : olfactory epithelium tissue
Blocking : 5% FBS, 25°C, 1Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : citric acid, pH6
Primary Antibody : 1:200, 4°C, 20Hr
Figure Note : IHC analysis of OCT-embedded rat olfactory epithelium,using GTX104124(green).
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Application Reference
Takayasu Moroki et al. J Toxicol Pathol 2021; 34 (2):161-180 Databases for technical aspects of immunohistochemistry: 2021 update.
实验应用 : IHC
种属反应 : Rat
Chongfa Tang et al. Sci Rep 2021; 11 (1):5848 Primary ectocervical epithelial cells display lower permissivity to Chlamydia trachomatis than HeLa cells and a globally higher pro-inflammatory profile.
实验应用 : ICC/IF
种属反应 : Human
Kun-Ling Lin et al. Int J Mol Sci 2021; 22 (17) Low-Intensity Extracorporeal Shock Wave Therapy Promotes Bladder Regeneration and Improves Overactive Bladder Induced by Ovarian Hormone Deficiency from Rat Animal Model to Human Clinical Trial.
实验应用 : WB, ICC/IF
种属反应 : Rat
Kim JY et al. Nat Commun 2019; 10 (1):3694 Priming mobilization of hair follicle stem cells triggers permanent loss of regeneration after alkylating chemotherapy.
实验应用 : IHC-Fr
种属反应 : Human
D'Amico S et al. Genes Dev 2018; 32 (17-18):1175-1187 STAT3 is a master regulator of epithelial identity and KRAS-driven tumorigenesis.
Wang S et al. Med Sci (Paris) 2018; 34 Focus issue F1:94-98 Knock-down of filaggrin influences the mitogen-activated protein kinases signaling pathway in normal human epidermal keratinocytes.
实验应用 : WB
种属反应 : Human
Fan JR et al. Cell Death Dis 2018; 9 (5):502 Potential role of CBX7 in regulating pluripotency of adult human pluripotent-like olfactory stem cells in stroke model.
实验应用 : IHC-Fr
种属反应 : Human
Lee YL et al. Am J Pathol 2017; 187 (9):1945-1959 elucidating mechanisms of bladder repair after hyaluronan instillation in *-induced ulcerative cystitis in animal model.
实验应用 : WB, IHC
种属反应 : Rat
Dang NN et al. Braz J Med Biol Res 2015; 48 (1):39-45 Filaggrin silencing by shRNA directly impairs the skin barrier function of normal human epidermal keratinocytes and then induces an immune response.
Shen CH et al. Mol Med Rep 2015; 11 (2):887-95 biological effect of * in urothelial cell lines and global gene expression analysis in the bladders of *-injected mice.
实验应用 : IHC
种属反应 : Human
Igawa K et al. Stem Cells Transl Med 2014; 3 (9):992-1001 Removal of reprogramming transgenes improves the tissue reconstitution potential of keratinocytes generated from human induced pluripotent stem cells.
实验应用 : ICC/IF
种属反应 : Human
Dang N et al. Mol Biol Rep 2014; Knockdown of filaggrin influences the epidermal terminal differentiation via MAPK pathway in normal human epidermal keratinocytes.
实验应用 : WB
种属反应 : Human
Wang S et al. J Biol Chem 2013; 288 (36):26265-74 Progesterone receptor A stability is mediated by glycogen synthase kinase-3β in the Brca1-deficient mammary gland.
实验应用 : WB
种属反应 : Human
Hassiotou F et al. Front Oncol 2013; 3:79 Expression of the Pluripotency Transcription Factor OCT4 in the Normal and Aberrant Mammary Gland.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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