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Cytokeratin 18抗体 [1E1]

Overexpression
Anti-Cytokeratin 18 antibody [1E1] used in Western Blot (WB). GTX84256
Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256
Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256
Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256
Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256
Anti-Cytokeratin 18 antibody [1E1] used in Western Blot (WB). GTX84256
Anti-Cytokeratin 18 antibody [1E1] used in Western Blot (WB). GTX84256
Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256
Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256
Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256
Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256
Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256
Anti-Cytokeratin 18 antibody [1E1] used in Western Blot (WB). GTX84256
Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256
Anti-Cytokeratin 18 antibody [1E1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84256
Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256
Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256
Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256

目录号 GTX84256

文献引用 1

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 1E1

同种型

 IgG1

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Monkey
产品包装
100 μl (¥7300)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:1000
ICC/IF 1:100
IHC-P 1:50
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

48 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 50% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Human recombinant protein fragment corresponding to amino acids 69-372 of human CK18 (NP_000215) produced in E.coli.

纯化方式

Purified by affinity chromatography
From tissue culture supernatant

偶联

Unconjugated

RRID

AB_10726718

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

keratin 18 , CK-18 , CYK18 , K18

细胞定位

Nucleolus

功能与背景

Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

数据库

研究领域

產品图片

Anti-Cytokeratin 18 antibody [1E1] used in Western Blot (WB). GTX84256

GTX84256 WB Image

Overexpression

WB analysis of HEK293T cells transfected with Cytokeratin 18 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84256 Cytokeratin 18 antibody [1E1].
Loading : 5 ug per lane

Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256

GTX84256 IHC-P Image

IHC-P analysis of human endometrium tissue using GTX84256 Cytokeratin 18 antibody [1E1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256

GTX84256 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX84256 Cytokeratin 18 antibody [1E1].
Antigen retrieval : Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120ººC for 3 min.
Dilution : 1:6000

Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256

GTX84256 IHC-P Image

IHC-P analysis of human liver tissue using GTX84256 Cytokeratin 18 antibody [1E1].
Antigen retrieval : Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120ººC for 3 min.
Dilution : 1:6000

Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256

GTX84256 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84256 Cytokeratin 18 antibody [1E1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Cytokeratin 18 antibody [1E1] used in Western Blot (WB). GTX84256

GTX84256 WB Image

WB analysis of various cell lines using GTX84256 Cytokeratin 18 antibody [1E1].
Loading : 35 ug per lane

Anti-Cytokeratin 18 antibody [1E1] used in Western Blot (WB). GTX84256

GTX84256 WB Image

WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX84256 Cytokeratin 18 antibody [1E1].
Loading : 10 ug per lane
Dilution : 1:200

Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256

GTX84256 IHC-P Image

IHC-P analysis of human colon tissue using GTX84256 Cytokeratin 18 antibody [1E1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256

GTX84256 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX84256 Cytokeratin 18 antibody [1E1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256

GTX84256 IHC-P Image

IHC-P analysis of human pancreas carcinoma tissue using GTX84256 Cytokeratin 18 antibody [1E1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256

GTX84256 IHC-P Image

IHC-P analysis of human kidney carcinoma tissue using GTX84256 Cytokeratin 18 antibody [1E1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256

GTX84256 IHC-P Image

IHC-P analysis of human kidney tissue using GTX84256 Cytokeratin 18 antibody [1E1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Cytokeratin 18 antibody [1E1] used in Western Blot (WB). GTX84256

GTX84256 WB Image

WB analysis of HepG2 cell lysate using GTX84256 Cytokeratin 18 antibody [1E1].
Dilution : 1:1000

Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256

GTX84256 IHC-P Image

IHC-P analysis of human breast tissue using GTX84256 Cytokeratin 18 antibody [1E1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Cytokeratin 18 antibody [1E1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84256

GTX84256 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with Cytokeratin 18 plasmid using GTX84256 Cytokeratin 18 antibody [1E1].

Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256

GTX84256 IHC-P Image

IHC-P analysis of human liver carcinoma tissue using GTX84256 Cytokeratin 18 antibody [1E1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256

GTX84256 IHC-P Image

IHC-P analysis of human colon adenocarcinoma tissue using GTX84256 Cytokeratin 18 antibody [1E1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256

GTX84256 IHC-P Image

IHC-P analysis of human prostate tissue using GTX84256 Cytokeratin 18 antibody [1E1].
Antigen retrieval : Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120ººC for 3 min.
Dilution : 1:6000

文献引用

Application Reference

Tong J et al. PLoS One 2018; 13 (10) : e0204077 Adipose-derived mesenchymal stem cells formed acinar-like structure when stimulated with breast epithelial cells in three-dimensional culture.
实验应用 : ICC/IF
种属反应 : Human
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¥ 7300
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