GeneTex中国官方网站
  • China

Cytokeratin 18抗体 [N1N3]

KO/KD
Anti-Cytokeratin 18 antibody [N1N3] used in Western Blot (WB). GTX112977
Anti-Cytokeratin 18 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX112977
Anti-Cytokeratin 18 antibody [N1N3] used in Western Blot (WB). GTX112977
Anti-Cytokeratin 18 antibody [N1N3] used in Western Blot (WB). GTX112977
Anti-Cytokeratin 18 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112977

目录号 GTX112977

文献引用 2

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 Cytokeratin 18 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

48 kDa. ( Note )

阳性对照

A549 , H1299 , HeLa

预测种属反应

Mouse, Bovine, Dog(>80% identity)

产品属性

形式

Liquid

存储溶液

0.1M Tris, 0.1M Glycine, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.83 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 18. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_10719880

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

keratin 18 , CK-18 , CYK18 , K18

细胞定位

Cytoplasm , perinuclear region

功能与背景

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Cytokeratin 18 antibody [N1N3] used in Western Blot (WB). GTX112977

GTX112977 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (15 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 18 antibody [N1N3] (GTX112977) diluted at 1:15000.

Anti-Cytokeratin 18 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX112977

GTX112977 IHC-P Image

Immunohistochemical analysis of paraffin-embedded hepatoma, using Cytokeratin 18 antibody(GTX112977) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cytokeratin 18 antibody [N1N3] used in Western Blot (WB). GTX112977

GTX112977 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 18 antibody [N1N3] (GTX112977) diluted at 1:5000.

Anti-Cytokeratin 18 antibody [N1N3] used in Western Blot (WB). GTX112977

GTX112977 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 18 antibody [N1N3] (GTX112977) diluted at 1:5000.

Anti-Cytokeratin 18 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112977

GTX112977 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Cytokeratin 18(GTX112977) antibody at 1:200 dilution.

文献引用

Application Reference

Salina ACG et al. Elife 2022; 11 Efferocytosis of SARS-CoV-2-infected dying cells impairs macrophage anti-inflammatory functions and clearance of apoptotic cells.
Huang HJ et al. Mol Biosyst 2014; (Epub) Proteomic analysis of rhein-induced cyt: ER stress mediates cell death in breast cancer cells.
实验应用 : WB
种属反应 : Human
我要回报文献

产品评论

目前没有使用 Cytokeratin 18 antibody [N1N3] (GTX112977) 的产品评论被收录。快发表第一篇产品评论吧！
我要评论产品
SDS
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
联系经销商
欣博盛生物科技有限公司
欣博盛生物科技有限公司
(全国热线) 4006-800-892 / (深圳) 0755-26755892 / (北京) 010-88594029 / (上海) 021-34613729 / (广州) 18024516375 / (香港) +852-69410778
http://www.neobioscience.com
genetex@neobioscience.com