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Cytokeratin 18抗体 [N3C3]

KO/KD
Anti-Cytokeratin 18 antibody [N3C3] used in Western Blot (WB). GTX112978
Anti-Cytokeratin 18 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112978
Anti-Cytokeratin 18 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112978
Anti-Cytokeratin 18 antibody [N3C3] used in Western Blot (WB). GTX112978
Anti-Cytokeratin 18 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112978
Anti-Cytokeratin 18 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112978
Anti-Cytokeratin 18 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112978
Anti-Cytokeratin 18 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112978
Anti-Cytokeratin 18 antibody [N3C3] used in Western Blot (WB). GTX112978
Anti-Cytokeratin 18 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112978
Anti-Cytokeratin 18 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112978
Anti-Cytokeratin 18 antibody [N3C3] used in IHC (Whole mount) (IHC-Wm). GTX112978
Anti-Cytokeratin 18 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112978

目录号 GTX112978

文献引用 1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IHC-Wm

种属反应

 Human, Mouse, Zebrafish, Dog
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 Cytokeratin 18 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

48 kDa. ( Note )

阳性对照

Raji , HeLa

预测种属反应

Rat, Bovine(>80% identity)

产品属性

形式

Liquid

存储溶液

0.1M Tris, 0.1M Glycine, 10% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 18. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1950644

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

keratin 18 , CK-18 , CYK18 , K18

细胞定位

Cytoplasm , perinuclear region

功能与背景

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Cytokeratin 18 antibody [N3C3] used in Western Blot (WB). GTX112978

GTX112978 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (15 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 18 antibody [N3C3] (GTX112978) diluted at 1:8000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cytokeratin 18 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112978

GTX112978 IHC-P Image

Cytokeratin 18 antibody [N3C3] detects Cytokeratin 18 protein at cytoplasm in mouse urinary bladder by immunohistochemical analysis.
Sample: Paraffin-embedded mouse urinary bladder.
Cytokeratin 18 antibody [N3C3] (GTX112978) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cytokeratin 18 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112978

GTX112978 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cytokeratin 18 (GTX112978) antibody at 1:200 dilution.

Anti-Cytokeratin 18 antibody [N3C3] used in Western Blot (WB). GTX112978

GTX112978 WB Image

Sample (30 μg of whole cell lysate)
A: Raji
10% SDS PAGE
GTX112978 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cytokeratin 18 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112978

GTX112978 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H661 xenograft, using Cytokeratin 18(GTX112978) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cytokeratin 18 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112978

GTX112978 IHC-P Image

Cytokeratin 18 antibody detects Cytokeratin 18 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog kidney.
Cytokeratin 18 stained by Cytokeratin 18 antibody (GTX112978) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cytokeratin 18 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112978

GTX112978 ICC/IF Image

Cytokeratin 18 antibody detects Cytokeratin 18 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cytokeratin 18 stained by Cytokeratin 18 antibody (GTX112978) diluted at 1:500.

Anti-Cytokeratin 18 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112978

GTX112978 IHC-P Image

Cytokeratin 18 antibody detects Cytokeratin 18 protein at cytoskeleton by immunohistochemical analysis.
Sample: Paraffin-embedded dog malignant nephroblastoma.
Cytokeratin 18 stained by Cytokeratin 18 antibody (GTX112978) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cytokeratin 18 antibody [N3C3] used in Western Blot (WB). GTX112978

GTX112978 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 18 antibody (GTX112978) diluted at 1:8000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Cytokeratin 18 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112978

GTX112978 IHC-P Image

Cytokeratin 18 antibody detects Cytokeratin 18 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog liver.
Cytokeratin 18 stained by Cytokeratin 18 antibody (GTX112978) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cytokeratin 18 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112978

GTX112978 IHC-P Image

Cytokeratin 18 antibody detects Cytokeratin 18 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog intestine.
Cytokeratin 18 stained by Cytokeratin 18 antibody (GTX112978) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cytokeratin 18 antibody [N3C3] used in IHC (Whole mount) (IHC-Wm). GTX112978

GTX112978 IHC-Wm Image

Immunohistochemical analysis (whole mount) of zebrafish embryo, using Cytokeratin 18 antibody [N3C3] (GTX112978) at 1:200 dilution.

Anti-Cytokeratin 18 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112978

GTX112978 IHC-P Image

Cytokeratin 18 antibody detects Cytokeratin 18 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog stomach.
Cytokeratin 18 stained by Cytokeratin 18 antibody (GTX112978) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

文献引用

Application Reference

Nguyen QH et al. Nat Commun 2018; 9 (1) : 2028 Profiling human breast epithelial cells using single cell RNA sequencing identifies cell diversity.
实验应用 : IHC-P
种属反应 : Human
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