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Cytokeratin 7抗体 [N1C2]

Orthogonal
Anti-Cytokeratin 7 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX109723
Anti-Cytokeratin 7 antibody [N1C2] used in IHC (Frozen sections) (IHC-Fr). GTX109723
Anti-Cytokeratin 7 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109723
Anti-Cytokeratin 7 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX109723
Anti-Cytokeratin 7 antibody [N1C2] used in Western Blot (WB). GTX109723

目录号 GTX109723

文献引用 7

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IHC-Fr

种属反应

 Human
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

51 kDa. ( Note )

预测种属反应

Mouse, Rat, Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.76 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 7. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1950654

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

keratin 7 , CK7 , K2C7 , K7 , SCL

细胞定位

Cytoplasm

功能与背景

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Cytokeratin 7 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX109723

GTX109723 IHC-P Image

Orthogonal

Immunohistochemical characterization of Synaptophysin (GTX100865), p63 (GTX102425) and Cytokeratin 7 (GTX109723) in human small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC) specimens.
Sample: Paraffin-embedded human SCLC (upper panel) and NSCLC (lower panel).
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 15 mins. The section was then incubated with primary antibody at 1:500 overnight at 4ºC and detected using an HRP conjugated avidin-biotin-peroxidase Complex system. DAB was used as the chromogen and counterstained with haematoxylin.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cytokeratin 7 antibody [N1C2] used in IHC (Frozen sections) (IHC-Fr). GTX109723

GTX109723 IHC-Fr Image

Immunohistochemical analysis of human placenta (paraformaldehyde-fixed frozen sections), using Cytokeratin 7(GTX109723) antibody at 1:200 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cytokeratin 7 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109723

GTX109723 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cytokeratin 7(GTX109723) antibody at 1:200 dilution.

Anti-Cytokeratin 7 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX109723

GTX109723 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using Cytokeratin 7(GTX109723) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cytokeratin 7 antibody [N1C2] used in Western Blot (WB). GTX109723

GTX109723 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 7 antibody [N1C2] (GTX109723) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

文献引用

Application Reference

Wuputra K et al. Int J Mol Sci 2022; 23 (14) FOXM1-CD44 Signaling Is Critical for the Acquisition of Regorafenib Resistance in Human Liver Cancer Cells.
Yen-Yun Wang et al. Cancers (Basel) 2020; 12 (12) Visfatin Enhances Breast Cancer Progression through CXCL1 Induction in Tumor-Associated Macrophages.
实验应用 : WB
种属反应 : Human
Chen LC et al. Biosens Bioelectron 2020; 155 : 112111 Improving the reproducibility, accuracy, and stability of an electrochemical biosensor platform for point-of-care use.
DaSilva-Arnold S et al. Mol Hum Reprod 2018; (Epub) heZEB2, a master regulator of the epithelial-mesenchymal transition, mediates trophoblast differentiation.
实验应用 : WB
种属反应 : Human
DaSilva-Arnold S et al. Biol Reprod 2018; (Epub) Human trophoblast epithelial-mesenchymal transition in abnormally invasive placenta.
实验应用 : WB
种属反应 : Human
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产品包装 报价 (¥)
¥ 4000
¥ 1700
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