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Cytokeratin 8抗体 [4D12]

KO/KD Overexpression
Anti-Cytokeratin 8 antibody [4D12] used in Western Blot (WB). GTX84248
Anti-Cytokeratin 8 antibody [4D12] used in Western Blot (WB). GTX84248
Anti-Cytokeratin 8 antibody [4D12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84248
Anti-Cytokeratin 8 antibody [4D12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84248
Anti-Cytokeratin 8 antibody [4D12] used in Western Blot (WB). GTX84248
Anti-Cytokeratin 8 antibody [4D12] used in IHC (Paraffin sections) (IHC-P). GTX84248

目录号 GTX84248

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 4D12

同种型

 IgG2a

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Dog, Monkey
产品包装
100 μl (¥7300)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000
ICC/IF 1:100
IHC-P 1:50
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

54 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 50% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Human recombinant protein fragment corresponding to amino acids 91-381 of human CK8 (NP_002264) produced in E.coli.

纯化方式

Purified by affinity chromatography
From tissue culture supernatant

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

keratin 8 , CARD2 , CK-8 , CK8 , CYK8 , K2C8 , K8 , KO

细胞定位

Cytoplasm,nucleoplasm

功能与背景

Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

数据库

研究领域

產品图片

Anti-Cytokeratin 8 antibody [4D12] used in Western Blot (WB). GTX84248

GTX84248 WB Image

Overexpression

WB analysis of HEK293T cells transfected with Cytokeratin 8 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84248 Cytokeratin 8 antibody [4D12].
Loading : 5 ug per lane

Anti-Cytokeratin 8 antibody [4D12] used in Western Blot (WB). GTX84248

GTX84248 WB Image

KO/KD

WB analysis of wild type and Cytokeratin 8 knock out HeLa cell lysate(10μg per lane) using GTX84248 Cytokeratin 8 antibody [4D12].
Dilution : 1:1000

Anti-Cytokeratin 8 antibody [4D12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84248

GTX84248 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with Cytokeratin 8 plasmid using GTX84248 Cytokeratin 8 antibody [4D12].

Anti-Cytokeratin 8 antibody [4D12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84248

GTX84248 ICC/IF Image

ICC/IF analysis of A549 cells using GTX84248 Cytokeratin 8 antibody [4D12].

Anti-Cytokeratin 8 antibody [4D12] used in Western Blot (WB). GTX84248

GTX84248 WB Image

WB analysis of various cell lines using GTX84248 Cytokeratin 8 antibody [4D12].
Loading : 35 ug per lane

Anti-Cytokeratin 8 antibody [4D12] used in IHC (Paraffin sections) (IHC-P). GTX84248

GTX84248 IHC-P Image

IHC-P analysis of human ovary tissue using GTX84248 Cytokeratin 8 antibody [4D12].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
产品包装 报价 (¥)
¥ 7300
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