产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
Mouse liver , Molt-4 , Raji
预测种属反应
Rat, Bovine, Pig, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
0.1M Tris, 0.1M Glycine, 10% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.53 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human Cytokeratin 8. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2037299
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
keratin 8 , CARD2 , CK-8 , CK8 , CYK8 , K2C8 , K8 , KO
细胞定位
Cytoplasm
功能与背景
This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX109489 WB Image
Sample (50 ug of whole cell lysate) A: mouse liver 10% SDS PAGE GTX109489 diluted at 1:1000
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GTX109489 WB Image
Sample (30μg whole cell lysate) A:MOLT4 (GTX27912) B:Raji (GTX27908) 10% SDS PAGE GTX109489 diluted at 1:1000
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GTX109489 ICC/IF Image
Cytokeratin 8 antibody detects Cytokeratin 8 protein at cytoplasm by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cytokeratin 8 stained by Cytokeratin 8 antibody (GTX109489) diluted at 1:500.
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GTX109489 IHC-Wm Image
Immunohistochemical analysis (whole mount) of zebrafish embryo, using Cytokeratin 8 antibody [N1N3] (GTX109489) at 1:200 dilution.
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GTX109489 IHC-P Image
Cytokeratin 8 antibody detects Cytokeratin 8 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded dog skin.Cytokeratin 8 stained by Cytokeratin 8 antibody (GTX109489) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX109489 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 8 antibody (GTX109489) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX109489 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cytokeratin 8(GTX109489) antibody at 1:200 dilution.
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GTX109489 IHC-P Image
Cytokeratin 8 antibody [N1N3] detects Cytokeratin 8 protein at cytoplasm and membrane in mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. Cytokeratin 8 antibody [N1N3] (GTX109489) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX109489 IHC-P Image
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using Cytokeratin 8(GTX109489) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献引用
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