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DARPP-32抗体

Anti-DARPP-32 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133350
Anti-DARPP-32 antibody used in IHC (Paraffin sections) (IHC-P). GTX133350
Anti-DARPP-32 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133350
Anti-DARPP-32 antibody used in IHC (Paraffin sections) (IHC-P). GTX133350
Anti-DARPP-32 antibody used in Western Blot (WB). GTX133350
Anti-DARPP-32 antibody used in IHC (Paraffin sections) (IHC-P). GTX133350

目录号 GTX133350

1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IHC-Fr

种属反应

 Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

22 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.27 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Mouse

免疫原

Recombinant protein encompassing a sequence within the C-terminus region of mouse DARPP-32. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2886947

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

protein phosphatase 1, regulatory inhibitor subunit 1B , AU040756 , DARPP-32 , Darpp32

细胞定位

Cytoplasm

功能与背景

Inhibitor of protein-phosphatase 1.

数据库

研究领域

產品图片

Anti-DARPP-32 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133350

GTX133350 ICC/IF Image

DARPP-32 antibody detects DARPP-32 protein at cell body by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DARPP-32 protein stained by DARPP-32 antibody (GTX133350) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Fluoroshield with DAPI (GTX30920).
Anti-DARPP-32 antibody used in IHC (Paraffin sections) (IHC-P). GTX133350

GTX133350 IHC-P Image

DARPP-32 antibody detects DARPP-32 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
DARPP-32 stained by DARPP-32 antibody (GTX133350) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-DARPP-32 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133350

GTX133350 IHC-Fr Image

DARPP-32 antibody detects DARPP-32 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: DARPP-32 protein stained by DARPP-32 antibody (GTX133350) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-DARPP-32 antibody used in IHC (Paraffin sections) (IHC-P). GTX133350

GTX133350 IHC-P Image

DARPP-32 antibody detects DARPP-32 protein at cytoplasm in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
DARPP-32 antibody (GTX133350) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-DARPP-32 antibody used in Western Blot (WB). GTX133350

GTX133350 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with DARPP-32 antibody (GTX133350) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DARPP-32 antibody used in IHC (Paraffin sections) (IHC-P). GTX133350

GTX133350 IHC-P Image

DARPP-32 antibody detects DARPP-32 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
DARPP-32 stained by DARPP-32 antibody (GTX133350) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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产品评论

DARPP-32 antibody 目录号 GTX133350

Rating
( Average 4 based on 1 users reviews)
实验应用
IHC (Frozen sections)(IHC-Fr)
( Average 4 based on 1 users reviews)
Anti-DARPP-32 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133350
Date : Anonymous submitted on 22-Mar-2018
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Sample : mouse brain (striatum)
Fixation : 4%PFA
Permeabilization /Antigen retrieval : PBST (0.3% TritonX-100)
Primary Antibody : 1:500, 25°C, 16Hr
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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