产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:10000 |
1:100-1:1000 |
1:100-1:1000 |
1:500-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
NIH-3T3 , Mouse brain , PC-12 , Rat2 , rat brain , 293T
预测种属反应
Bovine, Chicken, Xenopus laevis, Xenopus tropicalis(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 1% BSA, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.34 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the C-terminus region of human DDB1. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_1950102
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
damage specific DNA binding protein 1 , DDBA , UV-DDB1 , XAP1 , XPCE , XPE , XPE-BF
细胞定位
Cytoplasm , Nucleus
功能与背景
This gene encodes the large subunit of DNA damage-binding protein which is a heterodimer composed of a large and a small subunit. This protein functions in nucleotide-excision repair. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX100130 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDB1 antibody (GTX100130) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX100130 IHC-P Image
Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft , using DDB1(GTX100130) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX100130 IHC-P Image
DDB1 antibody detects DDB1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.DDB1 stained by DDB1 antibody (GTX100130) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX100130 IHC-P Image
DDB1 antibody detects DDB1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.DDB1 stained by DDB1 antibody (GTX100130) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX100130 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DDB1(GTX100130) antibody at 1:200 dilution.
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GTX100130 IHC-P Image
DDB1 antibody detects DDB1 protein at nucleus and cytosol on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. DDB1 antibody (GTX100130) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX100130 WB Image
Rat tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with DDB1 antibody (GTX100130) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX100130 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DDB1 antibody (GTX100130) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX100130 WB Image
Sample (50 μg of whole cell lysate) A: Mouse brain 5% SDS PAGE GTX100130 diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX100130 WB Image
Sample (30 μg of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE GTX100130 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX100130 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DDB1 antibody (GTX100130) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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