DDDDK tag antibody [GT231] immunoprecipitates DDDK-tagged protein in IP experiments.
IP Sample: 750 μg whole cell lysate/extract of DDDK-tagged protein expressing 293T cells.
A : 5 μg whole cell lysate/extract of DDDDK-tagged protein expressing 293T cells
B : Control with 2.5 μg of pre-immune mouse IgG
C : Immunoprecipitation of DDDK-tagged protein by 2.5 μg of DDDDK tag antibody [GT231] (GTX629631)
7.5% SDS-PAGE
The immunoprecipitated DDDK-tagged protein was detected by DDDDK tag antibody [GT231] (GTX629631) diluted at 1 : 5000.
EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.

DDDDK Tag antibody [GT231] detects DDDDK Tag protein by Western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg whole cell extract of DDDDK-tagged protein expressing 293T cells
12 % SDS-PAGE
DDDDK Tag antibody [GT231] (GTX629631) dilution: 1:5000