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DDDDK tag抗体 [GT482]

Anti-DDDDK tag antibody [GT482] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636631
Anti-DDDDK tag antibody [GT482] used in Western Blot (WB). GTX636631
Anti-DDDDK tag antibody [GT482] used in Immunoprecipitation (IP). GTX636631

目录号 GTX636631

宿主

Mouse

克隆

Monoclonal

克隆编号

GT482

同种型

IgG2b

实验应用

WB, ICC/IF, IP

种属反应

Species independent
产品包装
100 μl (¥4000),
25 μl (¥1700)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000-1:10000
ICC/IF Assay dependent
IP Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原

The immunogen used to generate this antibody corresponds to DDDDK tag

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

RRID

AB_2910016

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

產品图片

Anti-DDDDK tag antibody [GT482] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636631

GTX636631 ICC/IF Image

DDDDK tag antibody [GT482] detects DDDDK tag protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DDDDK tag stained by DDDDK tag antibody [GT482] (GTX636631) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-DDDDK tag antibody [GT482] used in Western Blot (WB). GTX636631

GTX636631 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DDDDK tag antibody [GT482] (GTX636631) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-DDDDK tag antibody [GT482] used in Immunoprecipitation (IP). GTX636631

GTX636631 IP Image

Immunoprecipitation of DDDDK-tag tagged protein from DDDDK-tag tagged protein transfected 293T whole cell extracts using 5 μg of DDDDK tag antibody [GT482] (GTX636631).
Western blot analysis was performed using DDDDK tag antibody [GT482] (GTX636631).
EasyBlot HRP-conjugated anti mouse IgG antibody (GTX221667-01).

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产品包装 报价 (¥)
¥ 4000
¥ 1700
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