产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1-2.5 μg/ml |
5-10 μg/ml |
4-8 μg |
1-2.5 μg/ml |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
产品属性
形式
Liquid
存储溶液
PBS, 1% BSA
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
~0.8 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原
FLAG; peptide sequence DYKDDDDK
纯化方式
Purified by affinity chromatography
偶联
Unconjugated
RRID
AB_380231
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
功能与背景
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.
研究领域
產品图片
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GTX73143 WB Image
WB analysis of HEK-293T cells over-expressing N-Terminal Flag tagged fusion protein using GTX73143 DDDDK tag antibody.
Lane 1 : 0.4 μg/mL antibody
Lane 2 : Secondary antibody only
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GTX73143 WB Image
WB analysis of HEK-293T cells over-expressing C-Terminal Flag tagged fusion protein using GTX73143 DDDDK tag antibody.
Lane 1 : 0.4 μg/mL antibody
Lane 2 : Secondary antibody only
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GTX73143 ICC/IF Image
ICC/IF analysis of HEK-293T cells over-expressing C-Terminal Flag tagged fusion protein using GTX73143 DDDDK tag antibody at 0.8 μg/mL(red) with DAPI(blue). Cells were fixed and permeabilized with methanol followed by acetone.
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GTX73143 ICC/IF Image
ICC/IF analysis of HEK-293T cells over-expressing N-Terminal Flag tagged fusion protein using GTX73143 DDDDK tag antibody at 1.6 μg/mL(red) with DAPI(blue).
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