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DDR2抗体 [C2C3], C-term

KO/KD Overexpression
Anti-DDR2 antibody [C2C3], C-term used in Western Blot (WB). GTX102651
Anti-DDR2 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102651
Anti-DDR2 antibody [C2C3], C-term used in Western Blot (WB). GTX102651
Anti-DDR2 antibody [C2C3], C-term used in Western Blot (WB). GTX102651
Anti-DDR2 antibody [C2C3], C-term used in Western Blot (WB). GTX102651
Anti-DDR2 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX102651

目录号 GTX102651

1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

97 kDa. ( Note )

预测种属反应

Bovine(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the Intracellular domain of human DDR2. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_10719878

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

discoidin domain receptor tyrosine kinase 2 , MIG20a , NTRKR3 , TKT , TYRO10 , WRCN

细胞定位

Membrane

功能与背景

Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation, and metabolism. In several cases the biochemical mechanism by which RTKs transduce signals across the membrane has been shown to be ligand induced receptor oligomerization and subsequent intracellular phosphorylation. This autophosphorylation leads to phosphorylation of cytosolic targets as well as association with other molecules, which are involved in pleiotropic effects of signal transduction. RTKs have a tripartite structure with extracellular, transmembrane, and cytoplasmic regions. This gene encodes a member of a novel subclass of RTKs and contains a distinct extracellular region encompassing a factor VIII-like domain. Alternative splicing in the 5' UTR results in multiple transcript variants encoding the same protein. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-DDR2 antibody [C2C3], C-term used in Western Blot (WB). GTX102651

GTX102651 WB Image

KO/KD

Wild-type (WT) and DDR2 (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DDR2 antibody [C2C3], C-term (GTX102651) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DDR2 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102651

GTX102651 ICC/IF Image

DDR2 antibody [C2C3], C-term detects DDR2 protein at cell membrane by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DDR2 stained by DDR2 antibody [C2C3], C-term (GTX102651) diluted at 1:250.
Red: Tau, an Axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-DDR2 antibody [C2C3], C-term used in Western Blot (WB). GTX102651

GTX102651 WB Image

U87-MG whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DDR2 antibody [C2C3], C-term (GTX102651) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DDR2 antibody [C2C3], C-term used in Western Blot (WB). GTX102651

GTX102651 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DDR2 antibody [C2C3], C-term (GTX102651) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DDR2 antibody [C2C3], C-term used in Western Blot (WB). GTX102651

GTX102651 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with DDR2 antibody [C2C3], C-term (GTX102651) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DDR2 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX102651

GTX102651 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using DDR2(GTX102651) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

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产品评论

DDR2 antibody [C2C3], C-term 目录号 GTX102651

Rating
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实验应用
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
Anti-DDR2 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX102651
Date : Anonymous submitted on 11-May-2023
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : human breast carcinoma tissues, human prostate carcinoma tissues
Blocking : 10% Goat serum, 25°C, 0.5Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : Tris-EDTA buffer (pH9)
Primary Antibody : 1:400 for breast, 1:250 or prostate, 4°C, 15Hr
我要评论产品
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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