产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
HeLa
产品说明
This antibody was raised against human DDR2 Intracellular domain.
预测种属反应
Bovine(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the Intracellular domain of human DDR2. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
RRID
AB_2909967
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
discoidin domain receptor tyrosine kinase 2 , MIG20a , NTRKR3 , TKT , TYRO10 , WRCN
细胞定位
Cell membrane
功能与背景
This gene encodes a member of the discoidin domain receptor subclass of the receptor tyrosine kinase (RTKs) protein family. RTKs play a key role in the communication of cells with their microenvironment. The encoded protein is a collagen-induced receptor that activates signal transduction pathways involved in cell adhesion, proliferation, and extracellular matrix remodeling. This protein is expressed in numerous cell types and may alos be involved in wound repair and regulate tumor growth and invasiveness. Mutations in this gene are the cause of short limb-hand type spondylometaepiphyseal dysplasia. [provided by RefSeq, Aug 2017]
数据库
研究领域
產品图片
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GTX636322 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDR2 antibody [HL1107] (GTX636322) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636322 WB Image
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDR2 antibody [HL1107] (GTX636322) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636322 IHC-P Image
DDR2 antibody [HL1107] detects DDR2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse heart.DDR2 stained by DDR2 antibody [HL1107] (GTX636322) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636322 IHC-P Image
DDR2 antibody [HL1107] detects DDR2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse muscle.DDR2 stained by DDR2 antibody [HL1107] (GTX636322) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636322 WB Image
U87-MG whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DDR2 antibody [HL1107] (GTX636322) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636322 IHC-P Image
DDR2 antibody [HL1107] detects DDR2 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.DDR2 stained by DDR2 antibody [HL1107] (GTX636322) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636322 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDR2 antibody [HL1107] (GTX636322) diluted at 1:500000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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