GeneTex中国官方网站
  • China

DDX3抗体 [N3C2], Internal

Orthogonal
Anti-DDX3 antibody [N3C2], Internal used in Western Blot (WB). GTX110614
Anti-DDX3 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110614
Anti-DDX3 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110614
Anti-DDX3 antibody [N3C2], Internal used in Western Blot (WB). GTX110614
Anti-DDX3 antibody [N3C2], Internal used in Western Blot (WB). GTX110614
Anti-DDX3 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110614
Anti-DDX3 antibody [N3C2], Internal used in Western Blot (WB). GTX110614

目录号 GTX110614

文献引用 4

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 DDX3 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

阳性对照

Human ESC , U87MG

预测种属反应

Rat, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human DDX3. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2036751

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

DEAD-box helicase 3 X-linked , DEAD-box helicase 3 Y-linked , CAP-Rf , DBX , DDX14 , DDX3 , HLP2 , MRX102 , DBY

细胞定位

Cytoplasm , Nucleus , Nucleus speckle , Mitochondrion outer membrane

数据库

研究领域

產品图片

Anti-DDX3 antibody [N3C2], Internal used in Western Blot (WB). GTX110614

GTX110614 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX3 antibody [N3C2], Internal (GTX110614) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DDX3 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110614

GTX110614 ICC/IF Image

DDX3 antibody [N3C2], Internal detects DDX3 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DDX3 stained by DDX3 antibody [N3C2], Internal (GTX110614) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-DDX3 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110614

GTX110614 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using DDX3(GTX110614) antibody at 1:200 dilution.

Anti-DDX3 antibody [N3C2], Internal used in Western Blot (WB). GTX110614

GTX110614 WB Image

Orthogonal

U87-MG whole cell and cytoplasm extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX3 antibody [N3C2], Internal (GTX110614) diluted at 1:2000.

Anti-DDX3 antibody [N3C2], Internal used in Western Blot (WB). GTX110614

GTX110614 WB Image

Sample (20 ug of whole cell lysate)
A: human ESC
7.5% SDS PAGE
GTX110614 diluted at 1:2000

Anti-DDX3 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110614

GTX110614 IHC-P Image

DDX3 antibody [N3C2], Internal detects DDX3 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
DDX3 stained by DDX3 antibody [N3C2], Internal (GTX110614) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-DDX3 antibody [N3C2], Internal used in Western Blot (WB). GTX110614

GTX110614 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX3 antibody [N3C2], Internal (GTX110614) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

文献引用

Application Reference

Sarah C Atkinson et al. Int J Mol Sci 2021; 22 (16) TRIM25 and DEAD-Box RNA Helicase DDX3X Cooperate to Regulate RIG-I-Mediated Antiviral Immunity.
实验应用 : WB
种属反应 : Human
Deschepper CF et al. Sci Rep 2020; 10 (1) : 14900 Regulatory effects of the Uty/Ddx3y locus on neighboring chromosome Y genes and autosomal mRNA transcripts in adult mouse non-reproductive cells.
实验应用 : WB
种属反应 : Mouse
Tsai WC et al. Appl Immunohistochem Mol Morphol 2018; 26 (4) : 263-267 Applying DDX3X Biomarker to Discriminate Atypical from Benign Meningiomas in Tissue Microarray.
实验应用 : IHC-P
种属反应 : Human
Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
实验应用 : WB
种属反应 : Human
我要回报文献

产品评论

目前没有使用 DDX3 antibody [N3C2], Internal (GTX110614) 的产品评论被收录。快发表第一篇产品评论吧！
我要评论产品
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
联系经销商
欣博盛生物科技有限公司
欣博盛生物科技有限公司
(全国热线) 4006-800-892 / (深圳) 0755-26755892 / (北京) 010-88594029 / (上海) 021-34613729 / (广州) 18024516375 / (香港) +852-69410778
http://www.neobioscience.com
genetex@neobioscience.com