产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:1000-1:10000 |
Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the C-terminus region of human DDX4. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
DEAD-box helicase 4 , VASA
细胞定位
Cytoplasm
功能与背景
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
数据库
研究领域
產品图片
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GTX636961 IHC-P Image
DDX4 antibody [HL1487] detects DDX4 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. DDX4 stained by DDX4 antibody [HL1487] (GTX636961) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636961 IHC-P Image
DDX4 antibody [HL1487] detects DDX4 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat testis.DDX4 stained by DDX4 antibody [HL1487] (GTX636961) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636961 WB Image
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX4 antibody [HL1487] (GTX636961) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636961 WB Image
Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX4 antibody [HL1487] (GTX636961) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636961 WB Image
Human tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX4 antibody [HL1487] (GTX636961) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636961 IHC-P Image
DDX4 antibody [HL1487] detects DDX4 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat testis.DDX4 stained by DDX4 antibody [HL1487] (GTX636961) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献引用
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产品评论
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