产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
U87-MG , PC-12 , HeLa
预测种属反应
Mouse, Bovine, Dog, Chicken, Pig, Xenopus laevis(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 1% BSA, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.66 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human DLD. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2036767
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
dihydrolipoamide dehydrogenase , DLDD , DLDH , E3 , GCSL , LAD , PHE3
细胞定位
Mitochondrion matrix
功能与背景
This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. [provided by RefSeq]
数据库
研究领域
產品图片
![Anti-DLD antibody [N1N3] used in Western Blot (WB). GTX101232](/templates/default/js/lightbox/images/loading.gif "Anti-DLD antibody [N1N3] used in Western Blot (WB). GTX101232")
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GTX101232 WB Image
Wild-type (WT) and DLD knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DLD antibody [N1N3] (GTX101232) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101232 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human hepatoma, using DLD(GTX101232) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101232 WB Image
Sample (30 ug of whole cell lysate)
A: U87-MG
10% SDS PAGE
GTX101232 diluted at 1:1000
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GTX101232 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DLD antibody [N1N3] (GTX101232) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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文献引用
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产品评论
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![Anti-DLD antibody [N1N3] used in Western Blot (WB). GTX101232](/upload/website/prouct_img/small/GTX101232/GTX101232_40667_20181102_WB_KO_watermark_w_23060100_670.webp "Anti-DLD antibody [N1N3] used in Western Blot (WB). GTX101232")
![Anti-DLD antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101232](/upload/website/prouct_img/small/GTX101232/GTX101232_40667_IHC_w_23060100_879.webp "Anti-DLD antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101232")
![Anti-DLD antibody [N1N3] used in Western Blot (WB). GTX101232](/upload/website/prouct_img/small/GTX101232/GTX101232_40667_WB_w_23060100_213.webp "Anti-DLD antibody [N1N3] used in Western Blot (WB). GTX101232")
![Anti-DLD antibody [N1N3] used in Western Blot (WB). GTX101232](/upload/website/prouct_img/small/GTX101232/GTX101232_40667_20181026_WB_R_w_23060100_154.webp "Anti-DLD antibody [N1N3] used in Western Blot (WB). GTX101232")
![Anti-DLD antibody [N1N3] used in Western Blot (WB). GTX101232](/upload/website/prouct_img/normal/GTX101232/GTX101232_40667_20181102_WB_KO_watermark_w_23060100_670.webp "Anti-DLD antibody [N1N3] used in Western Blot (WB). GTX101232")
![Anti-DLD antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101232](/upload/website/prouct_img/normal/GTX101232/GTX101232_40667_IHC_w_23060100_879.webp "Anti-DLD antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101232")
![Anti-DLD antibody [N1N3] used in Western Blot (WB). GTX101232](/upload/website/prouct_img/normal/GTX101232/GTX101232_40667_WB_w_23060100_213.webp "Anti-DLD antibody [N1N3] used in Western Blot (WB). GTX101232")
![Anti-DLD antibody [N1N3] used in Western Blot (WB). GTX101232](/upload/website/prouct_img/normal/GTX101232/GTX101232_40667_20181026_WB_R_w_23060100_154.webp "Anti-DLD antibody [N1N3] used in Western Blot (WB). GTX101232")
