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DLGAP1抗体

Anti-DLGAP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133264
Anti-DLGAP1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133264
Anti-DLGAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133264
Anti-DLGAP1 antibody used in Western Blot (WB). GTX133264
Anti-DLGAP1 antibody used in Western Blot (WB). GTX133264
Anti-DLGAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133264

目录号 GTX133264

宿主

Rabbit

克隆

Polyclonal

同种型

IgG

实验应用

WB, ICC/IF, IHC-P, IHC-Fr

种属反应

Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

110 kDa. ( Note )

阳性对照

mouse brain , rat brain , mouse hippocampus

预测种属反应

Human, Bovine(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.87 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Mouse

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse DLGAP1. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2886897

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

DLG associated protein 1 , 4933422O14Rik , 9630002F18 , AI845682 , AI848168 , BB075781 , D17Bwg0511e , GKAP/SAPAP , GKPA/SAPAP , Gkap , Sapap1 , mKIAA4162

细胞定位

Cell membrane; Peripheral membrane protein , Cell junction , synapse , postsynaptic cell membrane , postsynaptic density , synapse , Cell membrane , Cell junction , synapse , postsynaptic cell membrane , postsynaptic density , synapse

功能与背景

Part of the postsynaptic scaffold in neuronal cells.

数据库

研究领域

產品图片

Anti-DLGAP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133264

GTX133264 ICC/IF Image

DLGAP1 antibody detects DLGAP1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DLGAP1 stained by DLGAP1 antibody (GTX133264) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-DLGAP1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133264

GTX133264 IHC-Fr Image

DLGAP1 antibody detects DLGAP1 Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: DLGAP1 stained by DLGAP1 antibody (GTX133264) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-DLGAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133264

GTX133264 IHC-P Image

DLGAP1 antibody detects DLGAP1 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
DLGAP1 antibody (GTX133264) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-DLGAP1 antibody used in Western Blot (WB). GTX133264

GTX133264 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DLGAP1 antibody (GTX133264) diluted at 1:3000.

Anti-DLGAP1 antibody used in Western Blot (WB). GTX133264

GTX133264 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DLGAP1 antibody (GTX133264) diluted at 1:3000.
The observed M.W. is based on the publication: PMID: 21041448

Anti-DLGAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133264

GTX133264 IHC-P Image

DLGAP1 antibody detects DLGAP1 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
DLGAP1 antibody (GTX133264) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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