DLK/MAP3K12抗体

Name: DLK/MAP3K12抗体
Brand: GeneTex
SKU: GTX124127
Price: 4000.00 RMB
Rating: 5 (2 reviews)

Anti-DLK/MAP3K12 antibody used in IHC (Frozen sections) (IHC-Fr). GTX124127

Anti-DLK/MAP3K12 antibody used in Western Blot (WB). GTX124127

Anti-DLK/MAP3K12 antibody used in IHC (Paraffin sections) (IHC-P). GTX124127

目录号 GTX124127

文献引用 10

宿主	Rabbit
克隆	Polyclonal
同种型	IgG
实验应用	WB, IHC-P, IHC-Fr
种属反应	Human, Mouse, Rat
产品包装	100 μl (¥4000), 25 μl (¥1700)

查看所有 DLK/MAP3K12 的产品

View product citations for antibody GTX124127 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:500-1:3000
IHC-P	1:100-1:1000
IHC-Fr	1:100-1:1000

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

96 kDa. ( Note )

阳性对照

Helicobacter pylori

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Mouse

免疫原

Recombinant protein encompassing a sequence within the center region of mouse DLK/MAP3K12. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_11170703

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

mitogen-activated protein kinase kinase kinase 12 , DLK , MUK , Zpk

细胞定位

Cytoplasm , Membrane; Peripheral membrane protein

功能与背景

May be an activator of the JNK/SAPK pathway. Phosphorylates beta-casein, histone 1 and myelin basic protein in vitro.

数据库

Gene ID: 26404 Map3k12
UniProt: Q60700 Map3k12

研究领域

產品图片

GTX124127 IHC-Fr Image

DLK/MAP3K12 antibody detects DLK/MAP3K12 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: DLK/MAP3K12 protein stained by DLK/MAP3K12 antibody (GTX124127) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX124127 WB Image

Sample (50 μg of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX124127 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX124127 WB Image

DLK/MAP3K12 antibody detects DLK/MAP3K12 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DLK/MAP3K12 antibody (GTX124127) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX124127 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using MAP3K12(GTX124127) antibody at 1:250 dilution.

GTX124127 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DLK/MAP3K12 antibody (GTX124127) diluted at 1:1000.

GTX124127 WB Image

DLK/MAP3K12 antibody detects MAP3K12 protein by Western blot analysis.
A. 20 μg mouse embryonic stem cell whole cell lysate/extract
7.5 % SDS-PAGE
DLK/MAP3K12 antibody (GTX124127) dilution: 1:1000

GTX124127 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DLK/MAP3K12 antibody (GTX124127) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX124127 WB Image

The data was published in the journal Elife in 2019.PMID: 31042147

GTX124127 WB Image

The data was published in the journal Elife in 2019.PMID: 31042147

GTX124127 WB Image

The data was published in the journal Mol Neurodegener in 2019. PMID: 31775817

GTX124127 IHC-Fr Image

The data was published in the journal Mol Neurodegener in 2019. PMID: 31775817

文献引用

Application Reference

M Song et al. Ecotoxicol Environ Saf 2024; 276 : 116280 High-fat diet exacerbates 1-Bromopropane-induced loss of dopaminergic neurons in the substantia nigra of mice through mitochondrial damage associated necroptotic pathway

Duncan GJ et al. bioRxiv 2023; Remyelination protects neurons from DLK-mediated neurodegeneration.

K?ster KA et al. Naunyn Schmiedebergs Arch Pharmacol 2023; 396 (6) : 1223-1233 Increase of c-FOS promoter transcriptional activity by the dual leucine zipper kinase.

Lindborg JA et al. Cell Rep 2021; 34 (9) : 108777 Optic nerve regeneration screen identifies multiple genes restricting adult neural repair.

实验应用 : WB

Augert A et al. Cancer Cell 2020; 38 (1) : 97-114.e7 MAX Functions as a Tumor Suppressor and Rewires Metabolism in Small Cell Lung Cancer.

实验应用 : WB

种属反应 : Mouse

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产品评论

DLK/MAP3K12 antibody 目录号 GTX124127

Rating

( Average 5 based on 2 users reviews)

实验应用

Western Blot(WB)		( Average 5 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P)		( Average 5 based on 1 users reviews)

Date : Anonymous submitted on 20-Dec-2022

Score :

Application Tested : WB

Sample Species : Ms

Sample : mouse neural tissue

Amount used : 10ug

Blocking : BSA, 25°C, 2Hr

Primary Antibody : 1:1000, 4°C, 18Hr

Date : Anonymous submitted on 17-Nov-2022

Score :

Application Tested : IHC-P

Sample Species : Ms

Sample : brain-injured mouse (after 3days of injury)

Blocking : 0.5% skim milk in PBST, RT°C, 2Hr

Fixation : 4% PFA

Permeabilization /Antigen retrieval : PBS containing Triton X-100 (PBST)

Primary Antibody : 1:1000, 4°C, 18Hr

我要评论产品

Application Reference

M Song et al. Ecotoxicol Environ Saf 2024; 276:116280 High-fat diet exacerbates 1-Bromopropane-induced loss of dopaminergic neurons in the substantia nigra of mice through mitochondrial damage associated necroptotic pathway

Duncan GJ et al. bioRxiv 2023; Remyelination protects neurons from DLK-mediated neurodegeneration.

K?ster KA et al. Naunyn Schmiedebergs Arch Pharmacol 2023; 396 (6):1223-1233 Increase of c-FOS promoter transcriptional activity by the dual leucine zipper kinase.

Lindborg JA et al. Cell Rep 2021; 34 (9):108777 Optic nerve regeneration screen identifies multiple genes restricting adult neural repair.

实验应用 : WB

Augert A et al. Cancer Cell 2020; 38 (1):97-114.e7 MAX Functions as a Tumor Suppressor and Rewires Metabolism in Small Cell Lung Cancer.

实验应用 : WB

种属反应 : Mouse

Tenenbaum M et al. Cell Mol Life Sci 2020; The Map3k12 (Dlk)/JNK3 signaling pathway is required for pancreatic beta-cell proliferation during postnatal development.

种属反应 : Rat

Welsbie DS et al. Mol Neurodegener 2019; 14 (1):44 Targeted disruption of dual leucine zipper kinase and leucine zipper kinase promotes neuronal survival in a model of diffuse traumatic brain injury.

实验应用 : IHC-P

种属反应 : Mouse

Wang G et al. Elife 2019; 8 Structural plasticity of actin-spectrin membrane skeleton and functional role of actin and spectrin in axon degeneration.

实验应用 : WB

种属反应 : Mouse

Larhammar M et al. J Neurosci 2017; (Epub) The Ste20 family kinases MAP4K4, MINK1 and TNIK, converge to regulate stress induced JNK signaling in neurons.

实验应用 : WB

种属反应 : Mouse

Wu CC et al. Cell Cycle 2015; 14 (8):1207-17 Akt suppresses DLK for maintaining self-renewal of mouse embryonic stem cells.

实验应用 : WB

种属反应 : Mouse

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf

产品包装	报价 (¥)
100 μl	¥ 4000
25 μl	¥ 1700
Qty

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DLK/MAP3K12抗体

目录号 GTX124127

宿主

克隆

同种型

实验应用

种属反应

产品应用

应用说明

预期分子量

阳性对照

产品属性

形式

存储溶液

存放说明

浓度

免疫原种属

免疫原

纯化方式

偶联

RRID

注意事项

相关讯息

别名

细胞定位

功能与背景

数据库

研究领域

產品图片

GTX124127 IHC-Fr Image

GTX124127 WB Image

GTX124127 WB Image

GTX124127 IHC-P Image

GTX124127 WB Image

GTX124127 WB Image

GTX124127 WB Image

GTX124127 WB Image

GTX124127 WB Image

GTX124127 WB Image

GTX124127 IHC-Fr Image

文献引用

Application Reference

产品评论

DLK/MAP3K12 antibody 目录号 GTX124127

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