产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
293T , Molt-4 , NCI-H929
预测种属反应
Mouse, Rat, Dog(>80% identity)
产品属性
形式
Liquid
存储溶液
0.1M Tris, 0.1M Glycine, 10% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human DNA-PKcs. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_1951440
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
protein kinase, DNA-activated, catalytic subunit , DNA-PKC , DNA-PKcs , DNAPK , DNAPKc , DNPK1 , HYRC , HYRC1 , IMD26 , XRCC7 , p350
细胞定位
Nucleus
功能与背景
The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties.[supplied by OMIM]
数据库
研究领域
產品图片
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GTX109673 ICC/IF Image
DNA-PKcs antibody [C3], C-term detects DNA-PKcs protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DNA-PKcs protein stained by DNA-PKcs antibody [C3], C-term (GTX109673) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.
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GTX109673 WB Image
Sample(30 ug whole cell lysate) A:293T B:MOLT4 (GTX27912) 5% SDS PAGE GTX109673 diluted at 1:1000
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GTX109673 WB Image
DNA PKcs antibody detects DNA PKcs protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with DNA PKcs antibody (GTX109673) at a dilution of 1:500.
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GTX109673 IHC-P Image
DNA-PKcs antibody [C3], C-term detects DNA-PKcs protein at cytosol and nucleus on human ovarian carcinoma by immunohistochemical analysis. Sample: DNA-PKcs antibody [C3], C-term (GTX109673) dilution: 1:250.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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文献引用
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