产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
Rat2 , Molt-4 , 293T , A431 , HeLa , HepG2
预测种属反应
Mouse, Bovine(>80% identity)
产品属性
形式
Liquid
存储溶液
0.1M Tris, 0.1M Glycine, 10% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.56 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human DNA polymerase delta. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_1951307
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
DNA polymerase delta 1, catalytic subunit , CDC2 , CRCS10 , MDPL , POLD
细胞定位
Nucleus
功能与背景
The DNA polymerase delta complex is involved in DNA replication and repair, and it consists of the proliferating cell nuclear antigen (PCNA; MIM 176740), the multisubunit replication factor C (see MIM 102579), and the 4 subunit polymerase complex: POLD1, POLD2 (MIM 600815), POLD3 (MIM 611415), and POLD4 (MIM 611525) (Liu and Warbrick, 2006 [PubMed 16934752]).[supplied by OMIM]
数据库
研究领域
產品图片
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GTX102888 WB Image
DNA polymerase delta antibody detects POLD1 protein by Western blot analysis.
A. 30 μg Rat2 whole cell lysate/extract
5 % SDS-PAGE
DNA polymerase delta antibody (GTX102888) dilution: 1:1000
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GTX102888 IHC-P Image
DNA polymerase delta antibody detects DNA polymerase delta protein at cytoplasm and nucleus on human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
DNA polymerase delta antibody (GTX102888) diluted at 1:250.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX102888 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA polymerase delta antibody (GTX102888) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX102888 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DNA Polymerase delta, catalytic subunit(GTX102888) antibody at 1:200 dilution.
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GTX102888 WB Image
Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX102888 diluted at 1:3000
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文献引用
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