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DUSP8抗体 [HL2338]

Recombinant antibody KO/KD Orthogonal
Anti-DUSP8 antibody [HL2338] used in Western Blot (WB). GTX638541
Anti-DUSP8 antibody [HL2338] used in Western Blot (WB). GTX638541
Anti-DUSP8 antibody [HL2338] used in IHC (Paraffin sections) (IHC-P). GTX638541
Anti-DUSP8 antibody [HL2338] used in Western Blot (WB). GTX638541
Anti-DUSP8 antibody [HL2338] used in Western Blot (WB). GTX638541
Anti-DUSP8 antibody [HL2338] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638541
Anti-DUSP8 antibody [HL2338] used in Western Blot (WB). GTX638541

目录号 GTX638541

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL2338

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

66 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant fragment of human DUSP8

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

dual specificity phosphatase 8 , C11orf81 , HB5 , HVH-5 , HVH8

细胞定位

Cytoplasm,Nucleus

功能与背景

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates SAPK/JNK and p38, is expressed predominantly in the adult brain, heart, and skeletal muscle, is localized in the cytoplasm, and is induced by nerve growth factor and insulin. An intronless pseudogene for DUSP8 is present on chromosome 10q11.2. [provided by RefSeq, Jul 2008]

数据库

研究领域

產品图片

Anti-DUSP8 antibody [HL2338] used in Western Blot (WB). GTX638541

GTX638541 WB Image

KO/KD

Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DUSP8 antibody [HL2338] (GTX638541) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DUSP8 antibody [HL2338] used in Western Blot (WB). GTX638541

GTX638541 WB Image

Human tissue extract (5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DUSP8 antibody [HL2338] (GTX638541) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DUSP8 antibody [HL2338] used in IHC (Paraffin sections) (IHC-P). GTX638541

GTX638541 IHC-P Image

DUSP8 antibody [HL2338] detects DUSP8 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
DUSP8 stained by DUSP8 antibody [HL2338] (GTX638541) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-DUSP8 antibody [HL2338] used in Western Blot (WB). GTX638541

GTX638541 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DUSP8 antibody [HL2338] (GTX638541) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DUSP8 antibody [HL2338] used in Western Blot (WB). GTX638541

GTX638541 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DUSP8 antibody [HL2338] (GTX638541) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DUSP8 antibody [HL2338] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638541

GTX638541 ICC/IF Image

DUSP8 antibody [HL2338] detects DUSP8 protein at cytoplasm by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DUSP8 stained by DUSP8 antibody [HL2338] (GTX638541) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-DUSP8 antibody [HL2338] used in Western Blot (WB). GTX638541

GTX638541 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DUSP8 antibody [HL2338] (GTX638541) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

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产品包装 报价 (¥)
¥ 4000
¥ 1700
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