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Dengue virus Envelope protein抗体 [GT643]

Anti-Dengue virus Envelope protein antibody [GT643] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX629117
Anti-Dengue virus Envelope protein antibody [GT643] used in Western Blot (WB). GTX629117
Anti-Dengue virus Envelope protein antibody [GT643] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629117
Anti-Dengue virus Envelope protein antibody [GT643] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629117
Anti-Dengue virus Envelope protein antibody [GT643] used in Western Blot (WB). GTX629117
Anti-Dengue virus Envelope protein antibody [GT643] used in Western Blot (WB). GTX629117

目录号 GTX629117

文献引用 7

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 GT643

同种型

 IgG2b

实验应用

 WB, ICC/IF, FACS, IHC-P (cell pellet)

种属反应

 Dengue virus 2, Dengue virus 4
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:2000
FACS Assay dependent
IHC-P (cell pellet) Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

51-60 kDa. ( Note )

阳性对照

Dengue virus type 2 infected BHK21 cells

产品说明

The validated Dengue virus serotype for each application may differ. Please refer to the validated data. This antibody does not cross-react with YFV, JEV, Zika, and CHIKV Envelope protein.

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Dengue virus 2

免疫原

Recombinant protein encompassing a sequence within the center region of Dengue virus Envelope protein (Dengue virus 2 (strain 16681 PDK 53)). The exact sequence is proprietary.

纯化方式

Affinity purified by Protein G.

偶联

Unconjugated

RRID

AB_2888099

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

Dengue Virus Envelope , Dengue Virus E , DENV Envelope , DENV E

研究领域

產品图片

Anti-Dengue virus Envelope protein antibody [GT643] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX629117

GTX629117 IHC-P (cell pellet) Image

Dengue virus Envelope protein antibody [GT643] detects Dengue virus Envelope protein protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dengue virus BHK-21.
Red: Dengue virus Envelope protein stained by Dengue virus Envelope protein antibody [GT643] (GTX629117) diluted at 1:2000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Dengue virus Envelope protein antibody [GT643] used in Western Blot (WB). GTX629117

GTX629117 WB Image

Envelop protein (Dengue virus 2) antibody [GT643] detects Envelop protein (Dengue virus 2) protein by western blot analysis.
A. 30 μg BHK21 whole cell lysate/extract
B. 30 μg whole cell lysate/extract of Dengue virus type 2 infected BHK21 cells
C. 30 μg whole cell lysate/extract of Japanese encephalitis virus infected BHK21 cells
12% SDS-PAGE
Envelop protein (Dengue virus 2) antibody [GT643] (GTX629117) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Dengue virus Envelope protein antibody [GT643] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629117

GTX629117 ICC/IF Image

Envelope protein (Dengue virus) antibody [GT643] detects Envelope protein (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in paraformaldehyde.
Green: Envelope protein (Dengue virus) protein stained by Envelope protein (Dengue virus) antibody [GT643] (GTX629117) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Dengue virus Envelope protein antibody [GT643] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629117

GTX629117 ICC/IF Image

Immunofluorescent analysis of Dengue virus infected cells using Dengue virus Envelope protein antibody [GT643] antibody (GTX629117).
Sample: Multiple virus infected cells slide.
Green: Dengue virus Envelope protein antibody [GT643] antibody (GTX629117) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Dengue virus Envelope protein antibody [GT643] used in Western Blot (WB). GTX629117

GTX629117 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Dengue virus Envelope protein antibody [GT643] (GTX629117) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Dengue virus Envelope protein antibody [GT643] used in Western Blot (WB). GTX629117

GTX629117 WB Image

Non-infected (–) and infected (+) BHK-21 whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Dengue virus Envelope protein antibody [GT643] (GTX629117) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

文献引用

Application Reference

Wu KX et al. Acta Pharm Sin B 2023; 13 (5) : 2039-2055 The host-targeting compound peruvoside has a broad-spectrum antiviral activity against positive-sense RNA viruses.
Patthara Kongsuphol et al. Commun Med (Lond) 2021; 1 : 46 A rapid simple point-of-care assay for the detection of SARS-CoV-2 neutralizing antibodies.
Kongsuphol P. et al. Communications Medicine 2021; (Epub) A rapid simple point-of-care assay for the detection of SARS-CoV-2 neutralizing antibodies.
Kabir MA et al. Sci Rep 2020; 10 (1) : 11906 An antibody panel for highly specific detection and differentiation of Zika virus.
Loe MWC et al. Antiviral Res 2020; 184 : 104954 Betulinic acid exhibits antiviral effects against dengue virus infection.
实验应用 : WB
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产品包装 报价 (¥)
¥ 4000
¥ 1700
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