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Dengue virus NS1 protein抗体

Anti-Dengue virus NS1 protein antibody used in Western Blot (WB). GTX124280
Anti-Dengue virus NS1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124280
Anti-Dengue virus NS1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124280
Anti-Dengue virus NS1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124280
Anti-Dengue virus NS1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124280
Anti-Dengue virus NS1 protein antibody used in Western Blot (WB). GTX124280
Anti-Dengue virus NS1 protein antibody used in Western Blot (WB). GTX124280
Anti-Dengue virus NS1 protein antibody used in IHC (Paraffin sections) (IHC-P). GTX124280
Anti-Dengue virus NS1 protein antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX124280

目录号 GTX124280

文献引用 22

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, FACS, IP, ELISA, IHC-P (cell pellet)

种属反应

 Dengue virus 2
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 Dengue virus NS1 protein 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:2000
IHC-P 1:100-1:1000
FACS Assay dependent
IP Assay dependent
ELISA Assay dependent
IHC-P (cell pellet) Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

40 kDa. ( Note )

阳性对照

Dengue virus type 1 infected BHK-21 , Dengue virus type 2 infected BHK-21 , Dengue virus type 3 infected BHK-21 , Dengue virus type 4 infected BHK-21

产品说明

This antibody was raised against Dengue virus 2 NS1 protein, which may cross react with NS1 protein of Dengue virus 1/3/4 but does not recognize with NS1 protein of JEV.

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.25 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Dengue virus 2

免疫原

Recombinant protein encompassing a sequence within the center region of Dengue virus NS1 protein (Dengue virus 2 (strain 16681 PDK 53)). The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_11171351

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

Dengue Virus Non-structural protein 1 , Dengue Virus NS1 , DENV Non-structural protein 1 , DENV NS1

研究领域

產品图片

Anti-Dengue virus NS1 protein antibody used in Western Blot (WB). GTX124280

GTX124280 WB Image

Sample (20 μg of whole cell lysate)
A: BHK-21
B: Dengue virus 2 infect BHK-21
C: JEV infect BHK-21
12% SDS PAGE
GTX124280 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dengue virus NS1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124280

GTX124280 ICC/IF Image

NS1 (Dengue virus) antibody detects NS1 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 4 (right) were fixed in MeOH.
Green: NS1 (Dengue virus) protein stained by NS1 (Dengue virus) antibody (GTX124280) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Dengue virus NS1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124280

GTX124280 ICC/IF Image

NS1 (Dengue virus) antibody detects NS1 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 1 (right) were fixed in MeOH.
Green: NS1 (Dengue virus) protein stained by NS1 (Dengue virus) antibody (GTX124280) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Dengue virus NS1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124280

GTX124280 ICC/IF Image

NS1 (Dengue virus) antibody detects NS1 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: NS1 (Dengue virus) protein stained by NS1 (Dengue virus) antibody (GTX124280) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Dengue virus NS1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124280

GTX124280 ICC/IF Image

NS1 (Dengue virus) antibody detects NS1 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: NS1 (Dengue virus) protein stained by NS1 (Dengue virus) antibody (GTX124280) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Dengue virus NS1 protein antibody used in Western Blot (WB). GTX124280

GTX124280 WB Image

NS1 (Dengue virus ) antibody detects NS1 (Dengue virus ) protein by Western blot analysis. Un-infected (-) and infected (+, DENV2 infection for ) BHK-21 whole cell extracts (20 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NS1 (Dengue virus ) antibody (GTX124280) diluted by 1:2000.

Anti-Dengue virus NS1 protein antibody used in Western Blot (WB). GTX124280

GTX124280 WB Image

NS1 (Dengue virus) antibody detects NS1 (Dengue virus) protein by western blot analysis.
A. 66 μg BHK-21 whole cell extract
B. 66 μg whole cell extract of Dengue virus type 1 infected BHK-21 cells
C. 20 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells
D. 10 μg whole cell extract of Dengue virus type 3 infected BHK-21 cells
E. 66 μg whole cell extract of Dengue virus type 4 infected BHK-21 cells
10% SDS-PAGE
NS1 (Dengue virus) antibody (GTX124280) dilution: 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The results may vary from different DENV strains, viral infection status, and culture conditions.
Reference: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9, J Infect Dis. 2011 Jun 15;203(12):1775-83.

Anti-Dengue virus NS1 protein antibody used in IHC (Paraffin sections) (IHC-P). GTX124280

GTX124280 IHC-P Image

Dengue virus NS1 protein antibody detects Dengue virus NS1 protein protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded BHK-21 infected with dengue virus.
Green: Dengue virus NS1 protein stained by Dengue virus NS1 protein antibody (GTX124280) diluted at 1:2000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Dengue virus NS1 protein antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX124280

GTX124280 IHC-P (cell pellet) Image

Dengue virus NS1 protein antibody detects Dengue virus NS1 protein protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dengue virus BHK-21.
Green: Dengue virus NS1 protein stained by Dengue virus NS1 protein antibody (GTX124280) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

文献引用

Application Reference

Baquero-P?rez B et al. Nat Commun 2024; 15 (1) : 1964 N(6)-methyladenosine modification is not a general trait of viral RNA genomes.
N Yu et al. bioRxiv 2024; Brain transcriptomics reveals features of the host response after dengue virus typeⅠinfection induced central nervous system injury in AGB6 mice
实验应用 : WB
SH Wang et al. Virulence 2023; 14 (1) : 2279355 Lambda-free light chain: A serum marker of dengue disease via NS3 protease-mediated antibody cleavage
实验应用 : WB
Shen TJ et al. JCI Insight 2022; 7 (21) Hyperglycemia exacerbates dengue virus infection by facilitating poly(A)-binding protein-mediated viral translation.
实验应用 : WB
Jhan MK et al. Cells 2021; 10 (11) Polarization of Type 1 Macrophages Is Associated with the Severity of Viral Encephalitis Caused by Japanese Encephalitis Virus and Dengue Virus.
实验应用 : WB
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Application Reference
Baquero-P?rez B et al. Nat Commun 2024; 15 (1):1964 N(6)-methyladenosine modification is not a general trait of viral RNA genomes.
N Yu et al. bioRxiv 2024; Brain transcriptomics reveals features of the host response after dengue virus typeⅠinfection induced central nervous system injury in AGB6 mice
实验应用 : WB
SH Wang et al. Virulence 2023; 14 (1):2279355 Lambda-free light chain: A serum marker of dengue disease via NS3 protease-mediated antibody cleavage
实验应用 : WB
Shen TJ et al. JCI Insight 2022; 7 (21) Hyperglycemia exacerbates dengue virus infection by facilitating poly(A)-binding protein-mediated viral translation.
实验应用 : WB
Jhan MK et al. Cells 2021; 10 (11) Polarization of Type 1 Macrophages Is Associated with the Severity of Viral Encephalitis Caused by Japanese Encephalitis Virus and Dengue Virus.
实验应用 : WB
Lin JJ et al. PLoS Pathog 2021; 17 (3):e1009480 Aggressive organ penetration and high vector transmissibility of epidemic dengue virus-2 Cosmopolitan genotype in a transmission mouse model.
实验应用 : WB
Shen TJ et al. J Immunol Res 2021; 2021:6680913 Antiviral Efficacy of the Anesthetic Propofol against Dengue Virus Infection and Cellular Inflammation.
实验应用 : WB
Shen TJ et al. Front Cell Infect Microbiol 2020; 10:557610 CNS Immune Profiling in a Dengue Virus-Infected Immunocompetent Outbred ICR Mice Strain.
实验应用 : WB
Hsieh TH et al. Front Cell Infect Microbiol 2020; 10:375 Senescence in Monocytes Facilitates Dengue Virus Infection by Increasing Infectivity.
实验应用 : WB
Rosales Ramirez R et al. J Virol 2019; 93 (4) The Dengue Virus Nonstructural Protein 1 (NS1) Is Secreted from Mosquito Cells in Association with the Intracellular Cholesterol Transporter Chaperone Caveolin Complex.
Kao JC et al. PLoS Negl Trop Dis 2018; 12 (8):e0006715 The antiparasitic drug niclosamide inhibits dengue virus infection by interfering with endosomal acidification independent of mTOR.
实验应用 : WB
Romel Rosales Ramirez et al. bioRxiv 2018; (Epub) The dengue virus non-structural protein 1 (NS1) is secreted from mosquito cells in association with the intracellular cholesterol transporter chaperone caveolin complex.
实验应用 : IP
Reid DW et al. J Virol 2018; (Epub) Dengue virus selectively annexes endoplasmic reticulum-associated translation machinery as a strategy for co-opting host cell protein synthesis.
实验应用 : WB
Hafirassou ML et al. Cell Rep 2017; 21 (13):3900-3913 A Global Interactome Map of the Dengue Virus NS1 Identifies Virus Restriction and Dependency Host Factors.
实验应用 : WB
Ho MR et al. Sci Rep 2017; 7 (1):6910 Blockade of dengue virus infection and viral cytotoxicity in neuronal cells in vitro and in vivo by targeting endocytic pathways.
实验应用 : WB
Sun P et al. J Immunol Methods 2016; (Epub) NK cell degranulation as a marker for measuring antibody-dependent cytotoxicity in neutralizing and non-neutralizing human sera from dengue patients.
实验应用 : FACS
Hsu YL et al. Sci Rep 2016; 6:24530 dengue virus infection induces *-lambda1 to facilitate cell migration.
Cheng YL et al. Sci Rep 2016; 6:32000 Activation of Nrf2 by the dengue virus causes an increase in CLEC5A, which enhances TNF-α production by mononuclear phagocytes.
实验应用 : WB
Chen HR et al. PLoS Negl Trop Dis 2016; 10 (7):e0004828 Dengue Virus Nonstructural Protein 1 Induces Vascular Leakage through Macrophage Migration Inhibitory Factor and Autophagy.
实验应用 : ELISA
Marceau C.D. et al. Nature 2016; 535 (7610):159-63 Genetic dissection of Flaviviridae host factors through genome-scale CRISPR screens.
实验应用 : WB
Sarathy VV et al. J Gen Virol 2015; 96 (10):3035-48 Characterization of lethal dengue virus type 4 (DENV-4) TVP-376 infection in mice lacking both IFN-α/β and IFN-γ receptors (AG129) and comparison with the DENV-2 AG129 mouse model.
实验应用 : ICC/IF
Cheng YL et al. Mediators Inflamm 2015; 2015 (Epub):274025 Dengue Virus Infection Causes the Activation of Distinct NF-κB Pathways for Inducible Nitric Oxide Synthase and TNF-α Expression in RAW264.7 Cells.
实验应用 : WB
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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