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Dengue virus NS3 protein抗体

Anti-Dengue virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124252
Anti-Dengue virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124252
Anti-Dengue virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124252
Anti-Dengue virus NS3 protein antibody used in Western Blot (WB). GTX124252
Anti-Dengue virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124252
Anti-Dengue virus NS3 protein antibody used in Western Blot (WB). GTX124252
Anti-Dengue virus NS3 protein antibody used in Western Blot (WB). GTX124252
Anti-Dengue virus NS3 protein antibody used in IHC (Paraffin sections) (IHC-P). GTX124252
Anti-Dengue virus NS3 protein antibody used in Western Blot (WB). GTX124252
Anti-Dengue virus NS3 protein antibody used in Western Blot (WB). GTX124252
Anti-Dengue virus NS3 protein antibody used in Western Blot (WB). GTX124252
Anti-Dengue virus NS3 protein antibody used in Western Blot (WB). GTX124252
Anti-Dengue virus NS3 protein antibody used in Western Blot (WB). GTX124252
Anti-Dengue virus NS3 protein antibody used in Western Blot (WB). GTX124252
Anti-Dengue virus NS3 protein antibody used in Western Blot (WB). GTX124252
Anti-Dengue virus NS3 protein antibody used in Western Blot and Immunoprecipitation (WB IP). GTX124252

目录号 GTX124252

宿主

Rabbit

克隆

Polyclonal

同种型

IgG

实验应用

WB, ICC/IF, IHC-P, FACS, IP, ELISA, IHC

种属反应

Dengue virus 2
产品包装
100 μl (¥4000),
25 μl (¥1700)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:2000
IHC-P Assay dependent
FACS Assay dependent
IP Assay dependent
ELISA Assay dependent
IHC Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

70 kDa. ( Note )

阳性对照

Dengue virus type 1 infected BHK-21 , Dengue virus type 2 infected BHK-21 , Dengue virus type 3 infected BHK-21 , Dengue virus type 4 infected BHK-21

产品说明

Based on internal testing results, this antibody may cross react with Dengue virus type 1/3/4.

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.15 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Dengue virus 2

免疫原

Recombinant protein encompassing a sequence within the center region of Dengue virus NS3 protein (Dengue virus 2 (strain 16681 PDK 53)). The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_11171668

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

Dengue Virus Non-structural protein 3 , Dengue Virus NS3 , DENV Non-structural protein 3 , DENV NS3

研究领域

產品图片

Anti-Dengue virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124252

GTX124252 ICC/IF Image

NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (GTX124252) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Anti-Dengue virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124252

GTX124252 ICC/IF Image

NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 4 (right) were fixed in MeOH.
Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (GTX124252) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Anti-Dengue virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124252

GTX124252 ICC/IF Image

NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 1 (right) were fixed in MeOH.
Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (GTX124252) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Anti-Dengue virus NS3 protein antibody used in Western Blot (WB). GTX124252

GTX124252 WB Image

NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein by western blot analysis.
A. 30 μg BHK-21 whole cell extract
B. 30 μg whole cell extract of Dengue virus type 1 infected BHK-21 cells
C. 30 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells
D. 30 μg whole cell extract of Dengue virus type 3 infected BHK-21 cells
E. 30 μg whole cell extract of Dengue virus type 4 infected BHK-21 cells
7.5% SDS-PAGE
NS3 (Dengue virus) antibody (GTX124252) dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The results may vary from different DENV strains, viral infection status, and culture conditions.
Reference: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9, J Infect Dis. 2011 Jun 15;203(12):1775-83.

Anti-Dengue virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124252

GTX124252 ICC/IF Image

NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (GTX124252) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Anti-Dengue virus NS3 protein antibody used in Western Blot (WB). GTX124252

GTX124252 WB Image

Non-infected (–) and infected (+) BHK-21 whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dengue virus NS3 protein antibody (GTX124252) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dengue virus NS3 protein antibody used in Western Blot (WB). GTX124252

GTX124252 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dengue virus NS3 protein antibody (GTX124252) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dengue virus NS3 protein antibody used in IHC (Paraffin sections) (IHC-P). GTX124252

GTX124252 IHC-P Image

The data was published in the journal Sci Rep in 2018. PMID: 29559699

Anti-Dengue virus NS3 protein antibody used in Western Blot (WB). GTX124252

GTX124252 WB Image

The data was published in the journal Front Cell Infect Microbiol in 2018. PMID: 30186771

Anti-Dengue virus NS3 protein antibody used in Western Blot (WB). GTX124252

GTX124252 WB Image

The data was published in the journal Viruses in 2020. PMID: 32121148

Anti-Dengue virus NS3 protein antibody used in Western Blot (WB). GTX124252

GTX124252 WB Image

The data was published in the journal PLoS Pathog in 2015. PMID: 26717518

Anti-Dengue virus NS3 protein antibody used in Western Blot (WB). GTX124252

GTX124252 WB Image

The data was published in the journal PLoS Pathog in 2015. PMID: 26717518

Anti-Dengue virus NS3 protein antibody used in Western Blot (WB). GTX124252

GTX124252 WB Image

The data was published in the journal PLoS Pathog in 2015. PMID: 26717518

Anti-Dengue virus NS3 protein antibody used in Western Blot (WB). GTX124252

GTX124252 WB Image

The data was published in the journal Front Microbiol in 2019. PMID: 31824450

Anti-Dengue virus NS3 protein antibody used in Western Blot (WB). GTX124252

GTX124252 WB Image

The data was published in the journal Viruses in 2020. PMID: 32121148

Anti-Dengue virus NS3 protein antibody used in Western Blot and Immunoprecipitation (WB IP). GTX124252

GTX124252 WB IP Image

The data was published in the journal PLoS Pathog in 2015. PMID: 26717518

文献引用

产品评论

Dengue virus NS3 protein antibody 目录号 GTX124252

Rating ( Average 5 based on 1 users reviews)
实验应用
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 03-May-2021
Score :
Application Tested : WB
Sample : A549 cells
Amount used : 10 μg
Blocking : 5% skim milk in TBST, 27°C, 1Hr
Primary Antibody : 1:2000, 4°C, 12Hr
Secondary Antibody : GTX213110-01, 1:2000, 27°C, 2Hr
条带说明 : Non-infected (mock) and infected (infection) A549 whole cell extracts (10 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Dengue virus NS3 protein antibody (GTX124252) diluted at 1:2000.
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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