Dengue virus NS4B protein抗体

Name: Dengue virus NS4B protein抗体
Brand: GeneTex
SKU: GTX103349
Price: 4000.00 RMB
Rating: 5 (2 reviews)

Anti-Dengue virus NS4B protein antibody used in Western Blot (WB). GTX103349

Anti-Dengue virus NS4B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103349

目录号 GTX103349

文献引用 9

宿主	Rabbit
克隆	Polyclonal
同种型	IgG
实验应用	WB, ICC/IF, FACS
种属反应	Dengue virus 2
产品包装	100 μl (¥4000), 25 μl (¥1700)

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View product citations for antibody GTX103349 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:1000-1:10000
ICC/IF	1:100-1:2000
FACS	Assay dependent

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

27 kDa. ( Note )

阳性对照

Dengue virus type 2 infected BHK-21 , Dengue virus type 3 infected BHK-21 , Dengue virus 2-infected C6/36

产品说明

This antibody was raised against Dengue virus 2 NS4B protein, which may cross react with NS4B protein of Dengue virus 3.

产品属性

形式

Liquid

存储溶液

PBS, 40% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Dengue virus 2

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of Dengue virus NS4B protein (Dengue virus 2 (strain 16681 PDK 53)). The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1240700

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

Dengue Virus Non-structural protein 4B , Dengue Virus NS4B , DENV Non-structural protein 4B , DENV NS4B

研究领域

產品图片

GTX103349 WB Image

Sample(15 μg of cell lysate)
A: BHK21
B: DV infected BHK21
10% SDS PAGE
GTX103349 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103349 ICC/IF Image

NS4B (Dengue virus) antibody detects NS4B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: NS4B (Dengue virus) protein stained by NS4B (Dengue virus) antibody (GTX103349) diluted at 1:2000.
Blue: Hoechst 33342 staining.

GTX103349 ICC/IF Image

NS4B (Dengue virus) antibody detects NS4B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: NS4B (Dengue virus) protein stained by NS4B (Dengue virus) antibody (GTX103349) diluted at 1:2000.
Blue: Hoechst 33342 staining.

GTX103349 WB Image

NS4B (Dengue virus) antibody detects NS4B (Dengue virus) protein by western blot analysis.
A. 30 μg BHK-21 whole cell extrac
B. 30 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells
C. 30 μg whole cell extract of Dengue virus type 3 infected BHK-21 cells
12% SDS-PAGE
NS4B (Dengue virus) antibody (GTX103349) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The results may vary from different DENV strains, viral infection status, and culture conditions.
Reference: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9, J Infect Dis. 2011 Jun 15;203(12):1775-83.

GTX103349 ICC/IF Image

GTX103349 IF Image Immunofluorescence analysis of Denque virus 2-infected BHK-21, using Non-structural protein 4B (Dengue virus 2 ) antibody (GTX103349) at 1:2000 dilution.

GTX103349 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (15 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Dengue virus NS4B protein antibody (GTX103349) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103349 WB Image

The data was published in the journal Cell Rep in 2019.PMID: 31067454

GTX103349 ICC/IF Image

The data was published in the journal Viruses in 2019.PMID: 31461934

GTX103349 WB Image

The data was published in the journal Cell Rep in 2019.PMID: 31067454

文献引用

Application Reference

Udawatte DJ et al. Front Cell Infect Microbiol 2022; (12) : 926036 Dengue virus downregulates TNFR1- and TLR3-stimulated NF-κB activation by targeting RIPK1

实验应用 : WB

Shen TJ et al. JCI Insight 2022; 7 (21) Hyperglycemia exacerbates dengue virus infection by facilitating poly(A)-binding protein-mediated viral translation.

实验应用 : WB

Tian JN et al. Viruses 2019; 11 (9) A Dengue Virus Type 2 (DENV-2) NS4B-Interacting Host Factor

Lin DL et al. Cell Rep 2019; 27 (6) : 1666-1674.e4 The ER Membrane Protein Complex Promotes Biogenesis of Dengue and Zika Virus Non-structural Multi-pass Transmembrane Proteins to Support Infection.

实验应用 : WB, ICC/IF

Zhang J et al. Cell Host Microbe 2018; 23 (6) : 819-831.e5 Flaviviruses Exploit the Lipid Droplet Protein AUP1 to Trigger Lipophagy and Drive Virus Production.

实验应用 : WB, ICC/IF

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我要回报文献

产品评论

Dengue virus NS4B protein antibody 目录号 GTX103349

Rating

( Average 5 based on 2 users reviews)

实验应用

Western Blot(WB)		( Average 5 based on 1 users reviews)
Flow cytometry(FACS)		( Average 5 based on 1 users reviews)

Date : Anonymous submitted on 03-May-2021

Score :

Application Tested : WB

Sample : A549 cells

Amount used : 10 μg

Blocking : 5% skim milk in TBST, 27°C, 1Hr

Primary Antibody : 1:2000, 4°C, 12Hr

Secondary Antibody : GTX213110-01, 1:2000, 27°C, 2Hr

条带说明 : Non-infected (mock) and infected (infection) A549 whole cell extracts (10 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Dengue virus NS4B protein antibody (GTX103349) diluted at 1:2000.

Anti-Dengue virus NS4B protein antibody used in Flow cytometry (FACS). GTX103349

Date : Anonymous submitted on 15-Dec-2014

Score :

Application Tested : FACS

Sample Species : DENV2

Sample : Dengue virus type 2 infected huh7 cells

Fixation : 2% paraformaldehyde in PBS

Permeabilization /Antigen retrieval : 0.1% Saponin, 2% FBS in PBS

Primary Antibody : 1:500, 4°C, 16Hr

我要评论产品

Application Reference

Udawatte DJ et al. Front Cell Infect Microbiol 2022; (12):926036 Dengue virus downregulates TNFR1- and TLR3-stimulated NF-κB activation by targeting RIPK1

实验应用 : WB

Shen TJ et al. JCI Insight 2022; 7 (21) Hyperglycemia exacerbates dengue virus infection by facilitating poly(A)-binding protein-mediated viral translation.

实验应用 : WB

Tian JN et al. Viruses 2019; 11 (9) A Dengue Virus Type 2 (DENV-2) NS4B-Interacting Host Factor

Lin DL et al. Cell Rep 2019; 27 (6):1666-1674.e4 The ER Membrane Protein Complex Promotes Biogenesis of Dengue and Zika Virus Non-structural Multi-pass Transmembrane Proteins to Support Infection.

实验应用 : WB, ICC/IF

Zhang J et al. Cell Host Microbe 2018; 23 (6):819-831.e5 Flaviviruses Exploit the Lipid Droplet Protein AUP1 to Trigger Lipophagy and Drive Virus Production.

实验应用 : WB, ICC/IF

Perry JW et al. J Virol 2018; (Epub) Functional Analysis of the Dengue Virus Genome Using An Insertional Mutagenesis Screen.

实验应用 : WB

Barbier V et al. Virology 2017; 500:149-160 Dengue virus induces mitochondrial elongation through impairment of Drp1-triggered mitochondrial fission.

Hafirassou ML et al. Cell Rep 2017; 21 (13):3900-3913 A Global Interactome Map of the Dengue Virus NS1 Identifies Virus Restriction and Dependency Host Factors.

实验应用 : WB

Naik NG et al. J Virol 2015; 89 (13):6746-60 Mutation of Putative N-Glycosylation Sites on Dengue Virus NS4B Decreases RNA Replication.

实验应用 : WB

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf

产品包装	报价 (¥)
100 μl	¥ 4000
25 μl	¥ 1700
Qty

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	http://www.neobioscience.com
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Dengue virus NS4B protein抗体

目录号 GTX103349

宿主

克隆

同种型

实验应用

种属反应

产品应用

应用说明

预期分子量

阳性对照

产品说明

产品属性

形式

存储溶液

存放说明

浓度

免疫原种属

免疫原

纯化方式

偶联

RRID

注意事项

相关讯息

别名

研究领域

產品图片

GTX103349 WB Image

GTX103349 ICC/IF Image

GTX103349 ICC/IF Image

GTX103349 WB Image

GTX103349 ICC/IF Image

GTX103349 WB Image

GTX103349 WB Image

GTX103349 ICC/IF Image

GTX103349 WB Image

文献引用

Application Reference

产品评论

Dengue virus NS4B protein antibody 目录号 GTX103349

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