Dengue virus NS4B protein抗体

目录号 GTX124250

文献引用 21

宿主	Rabbit
克隆	Polyclonal
同种型	IgG
实验应用	WB, ICC/IF, FACS
种属反应	Dengue virus 2
产品包装	100 μl (¥4000), 25 μl (¥1700)

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View product citations for antibody GTX124250 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:500-1:3000
ICC/IF	1:100-1:2000
FACS	Assay dependent

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

27 kDa. ( Note )

阳性对照

Dengue virus type 2 infected BHK-21 cells , Dengue virus type 3 infected BHK-21 cells , Dengue virus 2-infected C6/36

产品说明

This antibody was raised against Dengue virus 2 NS4B protein, which may cross react with NS4B protein of Dengue virus 3 but does not recognize with NS4B protein of JEV.

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.88 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Dengue virus 2

免疫原

Full length Dengue virus 2 NS4B Recombinant protein (Dengue virus 2 (strain 16681 PDK 53))

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_11176998

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

Dengue Virus Non-structural protein 4B , Dengue Virus NS4B , DENV Non-structural protein 4B , DENV NS4B

研究领域

產品图片

GTX124250 ICC/IF Image

NS4B (Dengue virus) antibody detects NS4B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: NS4B (Dengue virus) protein stained by NS4B (Dengue virus) antibody (GTX124250) diluted at 1:2000.
Blue: Hoechst 33342 staining.

GTX124250 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed BHK-21 cell, using NS4B (Dengue virus 2)(GTX124250) antibody at 1:2000 dilution.

GTX124250 ICC/IF Image

NS4B (Dengue virus) antibody detects NS4B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: NS4B (Dengue virus) protein stained by NS4B (Dengue virus) antibody (GTX124250) diluted at 1:2000.
Blue: Hoechst 33342 staining.

GTX124250 WB Image

Sample (20 μg of whole cell lysate)
A: BHK-21
B: Dengue virus 2 infect BHK-21
C: JEV infect BHK-21
10% SDS PAGE
GTX124250 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX124250 WB Image

NS4B (Dengue virus) antibody detects NS4B (Dengue virus) protein by western blot analysis.
A. 30 μg BHK-21 whole cell extract
B. 30 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells
C. 30 μg whole cell extract of Dengue virus type 3 infected BHK-21 cells
12% SDS-PAGE
NS4B (Dengue virus) antibody (GTX124250) dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The results may vary from different DENV strains, viral infection status, and culture conditions.
Reference: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9, J Infect Dis. 2011 Jun 15;203(12):1775-83.

GTX124250 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (15 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Dengue virus NS4B protein antibody (GTX124250) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX124250 WB Image

The data was published in the journal PLoS Negl Trop Dis in 2014. PMID: 25412261

GTX124250 WB Image

The data was published in the journal PLoS Negl Trop Dis in 2014. PMID: 25412261

GTX124250 WB Image

The data was published in the journal PLoS Negl Trop Dis in 2014. PMID: 25412261

文献引用

Application Reference

Shwetha Shivaprasad et al. PLoS Pathog 2022; 18 (4) : e1010163 Loquacious modulates flaviviral RNA replication in mosquito cells.

实验应用 : WB

Mazeaud C et al. Viruses 2021; 13 (10) The Biogenesis of Dengue Virus Replication Organelles Requires the ATPase Activity of Valosin-Containing Protein.

实验应用 : WB

Sarah Goellner et al. STAR Protoc 2020; 1 (3) : 100173 Replication-Independent Generation and Morphological Analysis of Flavivirus Replication Organelles.

实验应用 : WB, ICC/IF

Loe MWC et al. Antiviral Res 2020; 184 : 104954 Betulinic acid exhibits antiviral effects against dengue virus infection.

实验应用 : WB

Cerikan B et al. Cell Rep 2020; 32 (1) : 107859 A Non-Replicative Role of the 3' Terminal Sequence of the Dengue Virus Genome in Membranous Replication Organelle Formation.

实验应用 : ICC/IF

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Application Reference

Shwetha Shivaprasad et al. PLoS Pathog 2022; 18 (4):e1010163 Loquacious modulates flaviviral RNA replication in mosquito cells.

实验应用 : WB

Mazeaud C et al. Viruses 2021; 13 (10) The Biogenesis of Dengue Virus Replication Organelles Requires the ATPase Activity of Valosin-Containing Protein.

实验应用 : WB

Sarah Goellner et al. STAR Protoc 2020; 1 (3):100173 Replication-Independent Generation and Morphological Analysis of Flavivirus Replication Organelles.

实验应用 : WB, ICC/IF

Loe MWC et al. Antiviral Res 2020; 184:104954 Betulinic acid exhibits antiviral effects against dengue virus infection.

实验应用 : WB

Cerikan B et al. Cell Rep 2020; 32 (1):107859 A Non-Replicative Role of the 3' Terminal Sequence of the Dengue Virus Genome in Membranous Replication Organelle Formation.

实验应用 : ICC/IF

Mohamed B et al. Sci Rep 2020; 10 (1):4040 Very-long-chain fatty acid metabolic capacity of 17-beta-hydroxysteroid dehydrogenase type 12 (HSD17B12) promotes replication of hepatitis C virus and related flaviviruses.

实验应用 : WB

Ngo AM et al. Elife 2019; 8 The ER membrane protein complex is required to ensure correct topology and stable expression of flavivirus polyproteins.

实验应用 : WB

Lee JK et al. Antiviral Res 2019; 171:104589 Antiviral activity of ST081006 against the dengue virus.

实验应用 : WB

P?aszczyca A et al. PLoS Pathog 2019; 15 (5):e1007736 A novel interaction between dengue virus nonstructural protein 1 and the NS4A-2K-4B precursor is required for viral RNA replication but not for formation of the membranous replication organelle.

Wesley Freppel et al. Bio Protoc 2018; 8 (24):e3115 Production, Titration and Imaging of Zika Virus in Mammalian Cells.

Scaturro P et al. Nature 2018; 561 (7722):253-257 An orthogonal proteomic survey uncovers novel Zika virus host factors.

实验应用 : WB

Reid DW et al. J Virol 2018; (Epub) Dengue virus selectively annexes endoplasmic reticulum-associated translation machinery as a strategy for co-opting host cell protein synthesis.

实验应用 : WB

Roth H et al. MBio 2017; 8 (1) Flavivirus Infection Uncouples Translation Suppression from Cellular Stress Responses.

实验应用 : ICC/IF

Hsieh MS et al. PLoS One 2017; 12 (11):e0188170 Detection and quantification of dengue virus using a novel biosensor system based on dengue NS3 protease activity.

实验应用 : ICC/IF

Sepulveda-Salinas KJ et al. Cell Stress Chaperones 2017; (Epub) Participation of dengue virus NS4B protein in the modulation of immune effectors dependent on ER stress in insect cells.

实验应用 : WB

Tsai TT et al. Antiviral Res 2017; 145:44-53 Targeting heat shock factor 1 as an antiviral strategy against dengue virus replication in-vitro and in-vivo.

实验应用 : WB

Ho MR et al. Sci Rep 2017; 7 (1):6910 Blockade of dengue virus infection and viral cytotoxicity in neuronal cells in vitro and in vivo by targeting endocytic pathways.

实验应用 : WB

Wu YW et al. Sci Rep 2016; 6:32243 Autophagy-associated dengue vesicles promote viral transmission avoiding antibody neutralization.

实验应用 : FACS

Fang YT et al. PLoS One 2014; 9 (10):e110655 Autophagy facilitates antibody-enhanced dengue virus infection in human pre-basophil/mast cells.

实验应用 : FACS

Tsai TT et al. PLoS Negl Trop Dis 2014; 8 (11):e3320 Antibody-dependent enhancement infection facilitates dengue virus-regulated signaling of IL-10 production in monocytes.

实验应用 : WB

Poh MK et al. Antiviral Res 2012; 93 (1):191-8 U18666A, an intra-cellular cholesterol transport inhibitor, inhibits dengue virus entry and replication.

实验应用 : WB

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf

产品包装	报价 (¥)
100 μl	¥ 4000
25 μl	¥ 1700
Qty

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Dengue virus NS4B protein抗体

目录号 GTX124250

宿主

克隆

同种型

实验应用

种属反应

产品应用

应用说明

预期分子量

阳性对照

产品说明

产品属性

形式

存储溶液

存放说明

浓度

免疫原种属

免疫原

纯化方式

偶联

RRID

注意事项

相关讯息

别名

研究领域

產品图片

GTX124250 ICC/IF Image

GTX124250 ICC/IF Image

GTX124250 ICC/IF Image

GTX124250 WB Image

GTX124250 WB Image

GTX124250 WB Image

GTX124250 WB Image

GTX124250 WB Image

GTX124250 WB Image

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