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Dengue virus NS5 protein抗体

Anti-Dengue virus NS5 protein antibody used in Western Blot (WB). GTX103350
Anti-Dengue virus NS5 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103350
Anti-Dengue virus NS5 protein antibody used in Western Blot (WB). GTX103350

目录号 GTX103350

文献引用 17

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF

种属反应

 Dengue virus 2
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

103 kDa. ( Note )

阳性对照

BHK-21 , Dengue virus type 2 infected BHK-21 cells , Dengue virus type 3 infected BHK-21 cells

产品说明

This antibody was raised against Dengue virus 2 NS5 protein, which may cross react with NS5 protein of Dengue virus 3.

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.19 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Dengue virus 2

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Dengue virus NS5 protein (Dengue virus 2 (strain 16681 PDK 53)). The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1240701

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

Dengue Virus Non-structural protein 5 , Dengue Virus NS5 , DENV Non-structural protein 5 , DENV NS5

研究领域

產品图片

Anti-Dengue virus NS5 protein antibody used in Western Blot (WB). GTX103350

GTX103350 WB Image

NS5 (Dengue virus) antibody detects NS5 (Dengue virus) protein by western blot analysis.
A. 30 μg BHK-21 whole cell extract
B. 30 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells
C. 30 μg whole cell extract of Dengue virus type 3 infected BHK-21 cells
7.5% SDS-PAGE
NS5 (Dengue virus) antibody (GTX103350) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The results may vary from different DENV strains, viral infection status, and culture conditions.
Reference: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9, J Infect Dis. 2011 Jun 15;203(12):1775-83.

Anti-Dengue virus NS5 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103350

GTX103350 ICC/IF Image

GTX103350 IF Image Immunofluorescence analysis of Denque virus 2-infected BHK-21, using Non-structural protein NS5 (Dengue virus 2 ) antibody (GTX103350) at 1:2000 dilution.

Anti-Dengue virus NS5 protein antibody used in Western Blot (WB). GTX103350

GTX103350 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (15 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Dengue virus NS5 protein antibody (GTX103350) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

文献引用

Application Reference

Pan P et al. Signal Transduct Target Ther 2023; 8 (1) : 194 SARS-CoV-2 N protein enhances the anti-apoptotic activity of MCL-1 to promote viral replication.
Chan C et al. bioRxiv 2023; Dengue virus exploits the host tRNA epitranscriptome to promote viral replication.
Wu Y et al. Front Microbiol 2022; 13 : 889693 Autophagy Activation Induces p62-Dependent Autophagic Degradation of Dengue Virus Capsid Protein During Infection.
Shwetha Shivaprasad et al. PLoS Pathog 2022; 18 (4) : e1010163 Loquacious modulates flaviviral RNA replication in mosquito cells.
实验应用 : WB
Weng SC et al. Parasit Vectors 2021; 14 (1) : 376 Blood glucose promotes dengue virus infection in the mosquito Aedes aegypti.
实验应用 : WB
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Application Reference
Pan P et al. Signal Transduct Target Ther 2023; 8 (1):194 SARS-CoV-2 N protein enhances the anti-apoptotic activity of MCL-1 to promote viral replication.
Chan C et al. bioRxiv 2023; Dengue virus exploits the host tRNA epitranscriptome to promote viral replication.
Wu Y et al. Front Microbiol 2022; 13:889693 Autophagy Activation Induces p62-Dependent Autophagic Degradation of Dengue Virus Capsid Protein During Infection.
Shwetha Shivaprasad et al. PLoS Pathog 2022; 18 (4):e1010163 Loquacious modulates flaviviral RNA replication in mosquito cells.
实验应用 : WB
Weng SC et al. Parasit Vectors 2021; 14 (1):376 Blood glucose promotes dengue virus infection in the mosquito Aedes aegypti.
实验应用 : WB
Aviner R et al. Nature 2021; Cotranslational prolyl hydroxylation is essential for flavivirus biogenesis.
实验应用 : WB
De Jes?s-Gonz?lez LA et al. Viruses 2020; 12 (6) The Nuclear Pore Complex: A Target for NS3 Protease of Dengue and Zika Viruses.
实验应用 : WB
Zhang J et al. Cell Host Microbe 2018; 23 (6):819-831.e5 Flaviviruses Exploit the Lipid Droplet Protein AUP1 to Trigger Lipophagy and Drive Virus Production.
实验应用 : WB
Roth H et al. MBio 2017; 8 (1) Flavivirus Infection Uncouples Translation Suppression from Cellular Stress Responses.
实验应用 : ICC/IF
Hafirassou ML et al. Cell Rep 2017; 21 (13):3900-3913 A Global Interactome Map of the Dengue Virus NS1 Identifies Virus Restriction and Dependency Host Factors.
实验应用 : WB
Reyes-Ruiz JM et al. Virology 2017; 515:74-80 Strand-like structures and the nonstructural proteins 5, 3 and 1 are present in the nucleus of mosquito cells infected with dengue virus.
实验应用 : ICC/IF
Marceau CD et al. Nature 2016; 535 (7610):159-63 Genetic dissection of Flaviviridae host factors through genome-scale CRISPR screens.
Lim SP et al. PLoS Pathog 2016; 12 (8):e1005737 Potent Allosteric Dengue Virus NS5 Polymerase Inhibitors: Mechanism of Action and Resistance Profiling.
实验应用 : ICC/IF
Marceau C.D. et al. Nature 2016; (Epub) Genetic dissection of Flaviviridae host factors through genome-scale CRISPR screens.
实验应用 : WB
Naik NG et al. J Virol 2015; 89 (13):6746-60 Mutation of Putative N-Glycosylation Sites on Dengue Virus NS4B Decreases RNA Replication.
实验应用 : WB
Fraser JE et al. Methods Mol Biol 2014; 1138:301-28 Investigating Dengue Virus Nonstructural Protein 5 (NS5) Nuclear Import.
de Wispelaere M et al. J Virol 2013; 87 (13):7367-81 the small molecules azd0530 and * inhibit dengue virus rna replication via fyn kinase.
实验应用 : WB
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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