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Dengue virus prM protein抗体

Anti-Dengue virus prM protein antibody used in Western Blot (WB). GTX128092
Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128092
Anti-Dengue virus prM protein antibody used in Western Blot (WB). GTX128092
Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128092
Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128092
Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128092
Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128092
Anti-Dengue virus prM protein antibody used in Western Blot (WB). GTX128092

目录号 GTX128092

文献引用 13
2

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF

种属反应

 Dengue virus 2
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 Dengue virus prM protein 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:20000
ICC/IF 1:100-1:2000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

22 kDa. ( Note )

阳性对照

Dengue virus type 1 infected BHK-21 , Dengue virus type 2 infected BHK-21 , Dengue virus type 3 infected BHK-21 , Dengue virus type 4 infected BHK-21

产品说明

This antibody was raised against Dengue virus 2 prM protein, which may cross react with prM protein of Dengue virus 1/3/4.

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.46 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Dengue virus 2

免疫原

Recombinant protein encompassing a sequence within the center region of Dengue virus PrM protein (Dengue virus 2 (strain 16681 PDK 53)). The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2885699

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

Dengue Virus Premembrane , Dengue Virus PrM , DENV Premembrane , DENV PrM

研究领域

產品图片

Anti-Dengue virus prM protein antibody used in Western Blot (WB). GTX128092

GTX128092 WB Image

Non-infected (–) and infected (+) Zebrafish embryos extracts (15 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Dengue virus prM protein antibody (GTX128092) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128092

GTX128092 ICC/IF Image

prM (Dengue virus) antibody detects prM (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: prM (Dengue virus) protein stained by prM (Dengue virus) antibody (GTX128092) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Dengue virus prM protein antibody used in Western Blot (WB). GTX128092

GTX128092 WB Image

prM (Dengue virus) antibody detects prM (Dengue virus) protein by western blot analysis.
A. 30 μg BHK-21 whole cell extract
B. 30 μg whole cell extract of Dengue virus type 1 infected BHK-21 cells
C. 30 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells
D. 30 μg whole cell extract of Dengue virus type 3 infected BHK-21 cells
E. 30 μg whole cell extract of Dengue virus type 4 infected BHK-21 cells
15% SDS-PAGE
prM (Dengue virus) antibody (GTX128092) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The results may vary from different DENV strains, viral infection status, and culture conditions.
Reference: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9, J Infect Dis. 2011 Jun 15;203(12):1775-83.

Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128092

GTX128092 ICC/IF Image

prM (Dengue virus) antibody detects prM (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: prM (Dengue virus) protein stained by prM (Dengue virus) antibody (GTX128092) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128092

GTX128092 ICC/IF Image

prM (Dengue virus) antibody detects prM (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 1 (right) were fixed in MeOH.
Green: prM (Dengue virus) protein stained by prM (Dengue virus) antibody (GTX128092) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128092

GTX128092 ICC/IF Image

prM (Dengue virus) antibody detects prM (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 4 (right) were fixed in MeOH.
Green: prM (Dengue virus) protein stained by prM (Dengue virus) antibody (GTX128092) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128092

GTX128092 ICC/IF Image

The data was published in the journal Front Microbiol in 2019. PMID: 31824450

Anti-Dengue virus prM protein antibody used in Western Blot (WB). GTX128092

GTX128092 WB Image

The data was published in the journal PLoS Negl Trop Dis in 2014. PMID: 25122182

文献引用

Application Reference

Pan P et al. Signal Transduct Target Ther 2023; 8 (1) : 194 SARS-CoV-2 N protein enhances the anti-apoptotic activity of MCL-1 to promote viral replication.
Pan P et al. Front Microbiol 2019; 10 : 2637 Dengue Virus Infection Activates Interleukin-1β to Induce Tissue Injury and Vascular Leakage.
Pan P et al. J Virol 2019; (Epub) Dengue Virus M Protein Promotes NLRP3 Inflammasome Activation to Induce Vascular Leakage in Mice.
实验应用 : WB
Utomo DIS et al. Mol Biotechnol 2019; (Epub) Formation of Virus-Like Particles of the Dengue Virus Serotype 2 Expressed in Silkworm Larvae.
实验应用 : WB
Ooi YS et al. Nat Microbiol 2019; An RNA-centric dissection of host complexes controlling flavivirus infection.
实验应用 : WB
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产品评论

Dengue virus prM protein antibody 目录号 GTX128092

Rating
( Average 3.5 based on 2 users reviews)
实验应用
Western Blot(WB)
( Average 3.5 based on 2 users reviews)
Anti-Dengue virus prM protein antibody used in Western Blot (WB). GTX128092
Date : Anonymous submitted on 25-Dec-2019
Score :
Application Tested : WB
Sample Species : DENV2
Sample : Huh7 cells
Amount used : 10 μg
Blocking : 2.5% non-fat skim milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
条带说明 : Lane 1: Huh7 whole cell lysate; Lane 2: Dengue virus type II NGC strain infected.
Anti-Dengue virus prM protein antibody used in Western Blot (WB). GTX128092
Date : Anonymous submitted on 04-Jun-2015
Score :
Application Tested : WB
Sample Species : DENV2
Sample : Dengue virus type 2 infected BHK21 cells
Amount used : 30μg
Blocking : 5% skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
条带说明 : Lane 1 and 2: BHK-21 cells. Lane 3 and 4: Dengue virus type 2 (PLO46) infected BHK-21 cells.
我要评论产品
Application Reference
Pan P et al. Signal Transduct Target Ther 2023; 8 (1):194 SARS-CoV-2 N protein enhances the anti-apoptotic activity of MCL-1 to promote viral replication.
Pan P et al. Front Microbiol 2019; 10:2637 Dengue Virus Infection Activates Interleukin-1β to Induce Tissue Injury and Vascular Leakage.
Pan P et al. J Virol 2019; (Epub) Dengue Virus M Protein Promotes NLRP3 Inflammasome Activation to Induce Vascular Leakage in Mice.
实验应用 : WB
Utomo DIS et al. Mol Biotechnol 2019; (Epub) Formation of Virus-Like Particles of the Dengue Virus Serotype 2 Expressed in Silkworm Larvae.
实验应用 : WB
Ooi YS et al. Nat Microbiol 2019; An RNA-centric dissection of host complexes controlling flavivirus infection.
实验应用 : WB
Puschnik AS et al. Cell Rep 2017; 21 (11):3032-3039 A Small-Molecule Oligosaccharyltransferase Inhibitor with Pan-flaviviral Activity.
实验应用 : WB
Chen M et al. Sci Rep 2017; 7 (1):15931 Broad-spectrum antiviral agents: secreted phospholipase A2 targets viral envelope lipid bilayers derived from the endoplasmic reticulum membrane.
Lin C et al. Antiviral Res 2017; (Epub) Design, synthesis, and biological evaluation of novel 7-deazapurine nucleoside derivatives as potential anti-dengue virus agents.
实验应用 : WB
Soto-Acosta R et al. PLoS Pathog 2017; 13 (4):e1006257 DENV up-regulates the HMG-CoA reductase activity through the impairment of AMPK phosphorylation: A potential antiviral target.
实验应用 : WB
Mukherjee S et al. Virology 2016; 497:33-40 Enhancing dengue virus maturation using a stable furin over-expressing cell line.
实验应用 : WB
Marceau C.D. et al. Nature 2016; 535 (7610):159-63 Genetic dissection of Flaviviridae host factors through genome-scale CRISPR screens.
实验应用 : WB
Lee CM et al. J Virol 2015; 89 (12):6171-83 Determinants of Dengue Virus NS4A Protein Oligomerization.
实验应用 : WB
Zhu X et al. PLoS Negl Trop Dis 2014; 8 (8):e3088 microrna-30e* suppresses dengue virus replication by promoting nf-κb-dependent ifn production.
实验应用 : WB
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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