Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Deptor antibody [N1C3] (GTX116540) diluted at 1:1000.

Sample (30 ug of whole cell lysate)
A: HCT116
10% SDS PAGE
GTX116540 diluted at 1:1000