产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:10000 |
| Assay dependent |
| 1:100-1:1000 |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
A549 , HCT116 , 50 μg mouse muscle , Rat2
预测种属反应
Rabbit, Bovine, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1.06 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human Desmin. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_1950112
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
desmin , CDCD3 , CMD1F , CSM1 , CSM2 , LGMD1D , LGMD1E , LGMD2R
细胞定位
Cytoplasm
功能与背景
This gene encodes a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX103557 WB Image
Desmin antibody detects Desmin protein by western blot analysis.
A. 30 μg Rat2 whole cell lysate/extract
10 % SDS-PAGE
Desmin antibody (GTX103557) dilution: 1:1000
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GTX103557 WB Image
Desmin antibody detects DES protein by Western blot analysis.
A. 50 μg mouse muscle lysate/extract
10 % SDS-PAGE
Desmin antibody (GTX103557) dilution: 1:10000
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GTX103557 WB Image
Desmin antibody detects DES protein by Western blot analysis.
A. 30 μg A549 whole cell lysate/extract | B. 30 μg HCT116 whole cell lysate/extract
B. 30 μg HCT116 whole cell lysate/extract
10 % SDS-PAGE
Desmin antibody (GTX103557) dilution: 1:3000
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GTX103557 IHC-P Image
Desmin antibody detects Desmin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat muscle.Desmin stained by Desmin antibody (GTX103557) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX103557 WB Image
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Desmin antibody (GTX103557) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX103557 IHC-P Image
Desmin antibody detects Desmin protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded dog muscle.Desmin stained by Desmin antibody (GTX103557) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX103557 ICC/IF Image
Desmin antibody detects Desmin protein at cytoplasm by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Desmin stained by Desmin antibody (GTX103557) diluted at 1:500.
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GTX103557 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Desmin antibody (GTX103557) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX103557 IHC-P Image
Desmin antibody detects Desmin protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded dog malignant soft tissue sarcoma.Desmin stained by Desmin antibody (GTX103557) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX103557 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Desmin antibody (GTX103557) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX103557 IHC-P Image
Desmin antibody detects Desmin protein at cytosol on mouse skeletal muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse skeletal muscle.
Desmin antibody (GTX103557) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX103557 IHC-P Image
Desmin antibody detects Desmin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse heart.Desmin stained by Desmin antibody (GTX103557) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX103557 IHC-P Image
Desmin antibody detects DES protein at cytosol on H1299 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded H1299 xenograft.
Desmin antibody (GTX103557) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX103557 IHC-P Image
Desmin antibody detects Desmin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse smooth muscle.Desmin stained by Desmin antibody (GTX103557) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX103557 WB Image
The data was published in the journal PLoS One in 2016.PMID: 27589391
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文献引用
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