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Desmoglein 2抗体 [N1N2], N-term

Orthogonal
Anti-Desmoglein 2 antibody [N1N2], N-term used in Western Blot (WB). GTX102508
Anti-Desmoglein 2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102508
Anti-Desmoglein 2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102508
Anti-Desmoglein 2 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102508
Anti-Desmoglein 2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102508
Anti-Desmoglein 2 antibody [N1N2], N-term used in Western Blot (WB). GTX102508

目录号 GTX102508

文献引用 1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

122 kDa. ( Note )

预测种属反应

Bovine(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.36 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the Extracellular domain of human Desmoglein 2. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1950146

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

desmoglein 2 , CDHF5 , HDGC

细胞定位

Cell membrane , Cell junction , desmosome

功能与背景

Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. This gene product is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This second family member is expressed in colon, colon carcinoma, and other simple and stratified epithelial-derived cell lines. Mutations in this gene have been associated with arrhythmogenic right ventricular dysplasia, familial, 10. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Desmoglein 2 antibody [N1N2], N-term used in Western Blot (WB). GTX102508

GTX102508 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Desmoglein 2 antibody [N1N2], N-term (GTX102508) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Desmoglein 2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102508

GTX102508 IHC-P Image

Immunohistochemical analysis of paraffin-embedded DU145 xenograft , using Desmoglein-2(GTX102508) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Desmoglein 2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102508

GTX102508 IHC-P Image

Desmoglein 2 antibody [N1N2], N-term detects Desmoglein 2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
Desmoglein 2 stained by Desmoglein 2 antibody [N1N2], N-term (GTX102508) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Desmoglein 2 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102508

GTX102508 ICC/IF Image

Desmoglein 2 antibody [N1N2], N-term detects Desmoglein 2 protein at cell membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Desmoglein 2 stained by Desmoglein 2 antibody [N1N2], N-term (GTX102508) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Desmoglein 2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102508

GTX102508 IHC-P Image

Desmoglein 2 antibody [N1N2], N-term detects Desmoglein 2 protein at cell membrane and cytoplasm in mouse skin by immunohistochemical analysis.
Sample: Paraffin-embedded mouse skin.
Desmoglein 2 antibody [N1N2], N-term (GTX102508) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Desmoglein 2 antibody [N1N2], N-term used in Western Blot (WB). GTX102508

GTX102508 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Desmoglein 2 antibody [N1N2], N-term (GTX102508) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

文献引用

Application Reference

Liu Y et al. Am J Physiol Gastrointest Liver Physiol 2017; 313 (5) : G478-G491 Kr?ppel-like factor 5 is essential for maintenance of barrier function in mouse colon.
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SDS
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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