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Doublecortin抗体

Comparison Overexpression
Anti-Doublecortin antibody used in Western Blot (WB). GTX134052
Anti-Doublecortin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134052
Anti-Doublecortin antibody used in Western Blot (WB). GTX134052
Anti-Doublecortin antibody used in Western Blot (WB). GTX134052
Anti-Doublecortin antibody used in IHC (Paraffin sections) (IHC-P). GTX134052
Anti-Doublecortin antibody used in IHC (Frozen sections) (IHC-Fr). GTX134052
Anti-Doublecortin antibody used in Western Blot (WB). GTX134052
Anti-Doublecortin antibody used in IHC (Paraffin sections) (IHC-P). GTX134052

目录号 GTX134052

文献引用 6

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IHC-Fr

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

41 kDa. ( Note )

阳性对照

mouse brain , mouse fetal brain , rat brain

预测种属反应

Bovine, Chicken(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.72 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Mouse

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse Doublecortin. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2887191

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

doublecortin , Dbct

细胞定位

Cytoplasm

功能与背景

This gene encodes a member of the doublecortin family. The protein encoded by this gene is a cytoplasmic protein and contains two doublecortin domains, which bind microtubules. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to direct neuronal migration by regulating the organization and stability of microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase. Studies in knockout mice lacking this gene and the LIS1 gene suggest that the molecular interaction of these two genes is important in both in neuronal migration and neurogenesis, and there is a cortical role of this gene in nuclear translocation and positioning of the mitotic spindle in radial glial mitotic division. Multiple transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Doublecortin antibody used in Western Blot (WB). GTX134052

GTX134052 WB Image

Comparison

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Doublecortin antibody (GTX134052) diluted at 1:1000 and competitor's antibody (MO22113) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.

Anti-Doublecortin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134052

GTX134052 ICC/IF Image

Doublecortin antibody detects Doublecortin protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Doublecortin stained by Doublecortin antibody (GTX134052) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Doublecortin antibody used in Western Blot (WB). GTX134052

GTX134052 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Doublecortin antibody (GTX134052) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Doublecortin antibody used in Western Blot (WB). GTX134052

GTX134052 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Doublecortin antibody (GTX134052) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Doublecortin antibody used in IHC (Paraffin sections) (IHC-P). GTX134052

GTX134052 IHC-P Image

Doublecortin antibody detects Doublecortin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse E10.5 mouse embryo.
Doublecortin stained by Doublecortin antibody (GTX134052) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Doublecortin antibody used in IHC (Frozen sections) (IHC-Fr). GTX134052

GTX134052 IHC-Fr Image

Doublecortin antibody detects Doublecortin protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse hippocampus.
Green: Doublecortin stained by Doublecortin antibody (GTX134052) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Doublecortin antibody used in Western Blot (WB). GTX134052

GTX134052 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Doublecortin antibody (GTX134052) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Doublecortin antibody used in IHC (Paraffin sections) (IHC-P). GTX134052

GTX134052 IHC-P Image

The data was published in the journal Int J Mol Sci in 2020.PMID: 32046281

文献引用

Application Reference

Chen M et al. Eur Respir J 2020; 56 (3) Elevated ACE-2 expression in the olfactory neuroepithelium: implications for anosmia and upper respiratory SARS-CoV-2 entry and replication.
Chen M et al. bioRxiv 2020; (Epub) Elevated ACE2 expression in the olfactory neuroepithelium: implications for anosmia and upper respiratory SARS-CoV-2 entry and replication.
Polis B et al. Int J Mol Sci 2020; 21 (3) Arginase Inhibition Supports Survival and Differentiation of Neuronal Precursors in Adult Alzheimer's Disease Mice.
Li A et al. Neurobiol Aging 2020; 86 : 81-91 Delivery of exogenous proteins by mesenchymal stem cells attenuates early memory deficits in a murine model of Alzheimer's disease.
实验应用 : IHC-P
种属反应 : Mouse
Haque MN et al. Nutr Neurosci 2018; 15 : 1-9 Stigmasterol promotes neuronal migration via reelin signaling in neurosphere migration assays.
实验应用 : ICC/IF
种属反应 : Rat
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PBS.pdf
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Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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