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E-Cadherin抗体 [DECMA-1]

Orthogonal
Anti-E-Cadherin antibody [DECMA-1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11512
Anti-E-Cadherin antibody [DECMA-1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11512
Anti-E-Cadherin antibody [DECMA-1] used in Western Blot (WB). GTX11512
Anti-E-Cadherin antibody [DECMA-1] used in Western Blot (WB). GTX11512
Anti-E-Cadherin antibody [DECMA-1] used in IHC (Frozen sections) (IHC-Fr). GTX11512
Anti-E-Cadherin antibody [DECMA-1] used in Western Blot (WB). GTX11512
Anti-E-Cadherin antibody [DECMA-1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11512
Anti-E-Cadherin antibody [DECMA-1] used in IHC (Paraffin sections) (IHC-P). GTX11512

目录号 GTX11512

文献引用 9

宿主

 Rat

克隆

 Monoclonal

克隆编号

 DECMA-1

同种型

 IgG1

实验应用

 WB, ICC/IF, IHC-P, IHC-Fr, IP

种属反应

 Human, Mouse, Bovine, Dog
产品包装
100 μl (¥6600)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB Assay dependent
ICC/IF 1:1,600
IHC-P Assay dependent
IHC-Fr Assay dependent
IP Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

98 kDa. ( Note )

阳性对照

MDCK

产品说明

Monoclonal Anti-Uvomorulin/E-Cadherin was selected against the mouse cell adhesion molecule uvomorulin/E-Cadherin. The antibody localizes the cell surface glycoprotein uvomorulin/E-cadherin that has been found to be identical to L-CAM, Cell CAM 80/120, and ARC-1. It blocks both the aggregation of mouse embryonal carcinoma cells and the compaction of pre-implantation embryos. The antibody disrupts confluent monolayers of Madin-Darby canine kidney (MDCK) epithelial cells. In indirect immunofluorescent staining of MDCK cells grown in culture, the antibody shows strong staining on the membrane of adjacent cells, after treatment with 0.5% Triton-X 100.

产品属性

形式

Liquid

存储溶液

Ascites

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

免疫原种属

Mouse

免疫原

mouse embryonal carcinoma cell line PCC4 Aza R1.

纯化方式

Unpurified

偶联

Unconjugated

RRID

AB_381324

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

cadherin 1 , AA960649 , ARC-1 , E-cad , Ecad , L-CAM , UVO , Um

细胞定位

Cell junction,Cell membrane; Single-pass type I membrane protein,Endosome

功能与背景

Cell-cell interactions during embryonic development and in tissue organization involve specific cell-adhesion molecules (CAMs) that are functionally defined by antibodies that interfere with cell-cell adhesion. CAMs are expressed on early embryonic cells and persist in derivatives of all three germ layers. The best characterized CAMs are N-CAM (neural CAM) and L-CAM (liver CAM). The protein Uvomorulin, initially identified in embryonal carcinoma, is identical to L-CAM, E-Cadherin, Cell CAM 80/120 and ARC-1, each of which have been characterized in different systems. E-Cadherin has been characterized as a 120 kDa cell surface glycoprotein from which an 84 kDa fragment can be released by trypsin digestion in the presence of Ca2+.

数据库

研究领域

產品图片

Anti-E-Cadherin antibody [DECMA-1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11512

GTX11512 ICC/IF Image

ICC/IF analysis of MDCK cells using GTX11512 E-Cadherin antibody [DECMA-1] at 1:3200. Cells were fixed and permeabilized with methanol followed by acetone.

Anti-E-Cadherin antibody [DECMA-1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11512

GTX11512 ICC/IF Image

ICC/IF analysis of MDCK cells using GTX11512 E-Cadherin antibody [DECMA-1] at 1:3,200. Cells were fixed and permeabilized with cold methanol followed by acetone.

Anti-E-Cadherin antibody [DECMA-1] used in Western Blot (WB). GTX11512

GTX11512 WB Image

WB analysis of MDCK cells using GTX11512 E-Cadherin antibody [DECMA-1] at 1:250.

Anti-E-Cadherin antibody [DECMA-1] used in Western Blot (WB). GTX11512

GTX11512 WB Image

Orthogonal

The data was published in the journal Int J Mol Sci in 2020.PMID: 31991851

Anti-E-Cadherin antibody [DECMA-1] used in IHC (Frozen sections) (IHC-Fr). GTX11512

GTX11512 IHC-Fr Image

Orthogonal

The data was published in the journal Int J Mol Sci in 2020.PMID: 31991851

Anti-E-Cadherin antibody [DECMA-1] used in Western Blot (WB). GTX11512

GTX11512 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2019. PMID: 31101909

Anti-E-Cadherin antibody [DECMA-1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11512

GTX11512 ICC/IF Image

The data was published in the journal Nat Commun in 2020.PMID: 31974352

Anti-E-Cadherin antibody [DECMA-1] used in IHC (Paraffin sections) (IHC-P). GTX11512

GTX11512 IHC-P Image

The data was published in the journal Sci Rep in 2019. PMID: 31101909

文献引用

Application Reference

Jooske L Monster et al. J Cell Biol 2021; 220 (5) An asymmetric junctional mechanoresponse coordinates mitotic rounding with epithelial integrity.
实验应用 : ICC/IF
种属反应 : Human
Wilson DH et al. Nat Commun 2020; 11 (1) : 445 Non-canonical Wnt signalling regulates scarring in biliary disease via the planar cell polarity receptors.
Li J et al. JCI Insight 2020; 5 (6) Renal protective effects of empagliflozin via inhibition of EMT and aberrant glycolysis in proximal tubules.
实验应用 : IHC-P
种属反应 : Mouse
Li S et al. Int J Mol Sci 2020; 21 (3) Deficiency in Dipeptidyl Peptidase-4 Promotes Chemoresistance through the CXCL12/CXCR4/mTOR/TGFβ Signaling Pathway in Breast Cancer Cells.
实验应用 : WB
种属反应 : Human
Takagaki Y et al. Sci Rep 2019; 9 (1) : 7519 Dipeptidyl peptidase-4 plays a pathogenic role in BSA-induced kidney injury in diabetic mice.
实验应用 : IHC-P
种属反应 : Mouse
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Application Reference
Jooske L Monster et al. J Cell Biol 2021; 220 (5) An asymmetric junctional mechanoresponse coordinates mitotic rounding with epithelial integrity.
实验应用 : ICC/IF
种属反应 : Human
Wilson DH et al. Nat Commun 2020; 11 (1):445 Non-canonical Wnt signalling regulates scarring in biliary disease via the planar cell polarity receptors.
Li J et al. JCI Insight 2020; 5 (6) Renal protective effects of empagliflozin via inhibition of EMT and aberrant glycolysis in proximal tubules.
实验应用 : IHC-P
种属反应 : Mouse
Li S et al. Int J Mol Sci 2020; 21 (3) Deficiency in Dipeptidyl Peptidase-4 Promotes Chemoresistance through the CXCL12/CXCR4/mTOR/TGFβ Signaling Pathway in Breast Cancer Cells.
实验应用 : WB
种属反应 : Human
Takagaki Y et al. Sci Rep 2019; 9 (1):7519 Dipeptidyl peptidase-4 plays a pathogenic role in BSA-induced kidney injury in diabetic mice.
实验应用 : IHC-P
种属反应 : Mouse
Hart KC et al. Proc Natl Acad Sci U S A 2017; 114 (29):E5845-E5853 E-cadherin and LGN align epithelial cell divisions with tissue tension independently of cell shape.
Gloerich M et al. Nat Commun 2017; 8:13996 Cell division orientation is coupled to cell-cell adhesion by the E-cadherin/LGN complex.
实验应用 : ICC/IF
种属反应 : Dog
Cheng F et al. Proc Natl Acad Sci U S A 2016; 113 (30):E4320-7 Vimentin coordinates fibroblast proliferation and keratinocyte differentiation in wound healing via TGF-β-Slug signaling.
实验应用 : WB
种属反应 : Mouse
Peppicelli S et al. Clin Exp Metastasis. 2014; 31 (4):423-33 Contribution of acidic melanoma cells undergoing epithelial-to-mesenchymal transition to aggressiveness of non-acidic melanoma cells.
实验应用 : WB
种属反应 : Mouse
SDS
Sodium Azide.pdf
产品包装 报价 (¥)
¥ 6600
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