产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
1:50-1:200 |
1:100-1:500 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
MCF-7
预测种属反应
Mouse, Rat, Bovine, Dog, Pig(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human E-Cadherin. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein G.
偶联
Unconjugated
RRID
AB_2888130
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
cadherin 1 , Arc-1 , BCDS1 , CD324 , CDHE , ECAD , LCAM , UVO
细胞定位
Cell junction , Cell membrane
功能与背景
This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX629692 WB Image
Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [GT311] (GTX629692) diluted at 1:2000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX629692 FACS Image
E-Cadherin antibody [GT311] (GTX629692) detects E-Cadherin protein by flow cytometry analysis. Sample: MCF-7 cell. Black: Unlabelled sample was used as a control. Red: E-Cadherin antibody [GT311] (GTX629692) dilution: 1:50. Acquisition of 20,000 events were collected for FACS analysis.
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GTX629692 IP Image
E-adherin antibody [GT311] immunoprecipitates E-adherin protein in IP experiments. IP samples: MCF-7 whole cell extract A. 30 μg MCF-7 cell whole cell extract B. Control with 3 μg of preimmune Mouse IgG C. Immunoprecipitation of E-adherin protein by 3 μg E-adherin antibody [GT311] (GTX629692) 5 % SDS-PAGE The immunoprecipitated E-adherin protein was detected by E-adherin antibody [GT311] (GTX629692) diluted at 1:500. [EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent]
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GTX629692 WB Image
E-cadherin antibody [GT311] detects E-cadherin protein by Western blot analysis. A. 30 μg MCF-7 whole cell lysate/extract B. 30 μg MDA-MB-231 whole cell lysate/extract 5 % SDS-PAGE E-cadherin antibody [GT311] (GTX629692) dilution: 1:1000
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