GeneTex中国官方网站
  • China

E-Cadherin抗体 [GT358]

KO/KD
Anti-E-Cadherin antibody [GT358] used in Western Blot (WB). GTX629694
Anti-E-Cadherin antibody [GT358] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629694
Anti-E-Cadherin antibody [GT358] used in Flow cytometry (FACS). GTX629694
Anti-E-Cadherin antibody [GT358] used in Western Blot (WB). GTX629694
Anti-E-Cadherin antibody [GT358] used in Western Blot (WB). GTX629694
Anti-E-Cadherin antibody [GT358] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629694

目录号 GTX629694

文献引用 1

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 GT358

同种型

 IgG2b

实验应用

 WB, ICC/IF, FACS

种属反应

 Human, Dog
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 E-Cadherin 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
FACS 1:50-1:200
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

97 kDa. ( Note )

阳性对照

MCF-7 , MDA-MB-231

预测种属反应

Mouse, Rat, Bovine, Pig(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human E-Cadherin. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein G.

偶联

Unconjugated

RRID

AB_2888131

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

cadherin 1 , Arc-1 , BCDS1 , CD324 , CDHE , ECAD , LCAM , UVO

细胞定位

Cell junction, Cell membrane,

功能与背景

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-E-Cadherin antibody [GT358] used in Western Blot (WB). GTX629694

GTX629694 WB Image

KO/KD

Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [GT358] (GTX629694) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-E-Cadherin antibody [GT358] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629694

GTX629694 ICC/IF Image

E-Cadherin antibody [GT358] detects E-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: MCF7 cells were fixed in ice-cold MeOH for 5 min.
Green: E-Cadherin protein stained by E-Cadherin antibody [GT358] (GTX629694) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-E-Cadherin antibody [GT358] used in Flow cytometry (FACS). GTX629694

GTX629694 FACS Image

E-Cadherin antibody [GT358] (GTX629694) detects E-Cadherin protein by flow cytometry analysis.
Sample: MCF-7 cell.
Black: Unlabelled sample was used as a control.
Red: E-Cadherin antibody [GT358] (GTX629694) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

Anti-E-Cadherin antibody [GT358] used in Western Blot (WB). GTX629694

GTX629694 WB Image

E-cadherin antibody [GT358] detects E-cadherin protein by Western blot analysis.
A. 30 μg MCF-7 whole cell lysate/extract
B. 30 μg MDA-MB-231 whole cell lysate/extract
7.5 % SDS-PAGE
E-cadherin antibody [GT358] (GTX629694) dilution: 1:1000

Anti-E-Cadherin antibody [GT358] used in Western Blot (WB). GTX629694

GTX629694 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [GT358] (GTX629694) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-E-Cadherin antibody [GT358] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629694

GTX629694 ICC/IF Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: E-Cadherin stained by E-Cadherin antibody (GTX629694) diluted at 1:500.

文献引用

Application Reference

Lin-Sheng Yu et al. Cancers (Basel) 2021; 13 (16) Tissue Architecture Influences the Biological Effectiveness of Boron Neutron Capture Therapy in In Vitro/In Silico Three-Dimensional Self-Assembly Cell Models of Pancreatic Cancers.
实验应用 : WB, ICC/IF
种属反应 : Human
我要回报文献

产品评论

目前没有使用 E-Cadherin antibody [GT358] (GTX629694) 的产品评论被收录。快发表第一篇产品评论吧！
我要评论产品
SDS
PBS.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
客制化偶联服务
联系经销商
欣博盛生物科技有限公司
欣博盛生物科技有限公司
(全国热线) 4006-800-892 / (深圳) 0755-26755892 / (北京) 010-88594029 / (上海) 021-34613729 / (广州) 18024516375 / (香港) +852-69410778
http://www.neobioscience.com
genetex@neobioscience.com