Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with E-Cadherin antibody [GT477] (GTX629691) diluted at 1:3000 and competitor's antibody diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [GT477] (GTX629691) diluted at 1:2000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

E-Cadherin antibody [GT477] detects E-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: MCF7 cells were fixed in ice-cold MeOH for 5 min.
Green: E-Cadherin protein stained by E-Cadherin antibody [GT477] (GTX629691) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [GT477] (GTX635000) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

E-Cadherin antibody [GT477] detects E-Cadherin protein at cell membrane and cytoplasm in rat intestine by immunohistochemical analysis.
Sample: Paraffin-embedded rat intestine.
E-Cadherin antibody [GT477] (GTX629691) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

E-adherin antibody [GT477] immunoprecipitates E-adherin protein in IP experiments.
IP samples: MCF-7 whole cell extract
A. Control with 3 μg of preimmune Mouse IgG
B. Immunoprecipitation of E-adherin protein by 3 μg E-adherin antibody [GT477] (GTX629691)
5 % SDS-PAGE
The immunoprecipitated E-adherin protein was detected by E-adherin antibody [GT477] (GTX629691) diluted at 1:500.
[EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent]

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [GT477] (GTX629691) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

E-Cadherin antibody [GT477] (GTX629691) detects E-Cadherin protein by flow cytometry analysis.
Sample: MCF-7 cell.
Black: Unlabelled sample was used as a control.
Red: E-Cadherin antibody [GT477] (GTX629691) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [GT477] (GTX629691) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

E-Cadherin antibody [GT477] detects E-Cadherin protein at cell membrane and cytoplasm in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
E-Cadherin antibody [GT477] (GTX629691) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

E-Cadherin antibody [GT477] detects E-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: E-Cadherin stained by E-Cadherin antibody [GT477] (GTX629691) diluted at 1:2000.

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [GT477] (GTX629691) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

E-Cadherin antibody [GT477] detects E-Cadherin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog skin.
E-Cadherin stained by E-Cadherin antibody [GT477] (GTX629691) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min