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E-Cadherin抗体 [HL1229]

Recombinant antibody KO/KD Orthogonal
Anti-E-Cadherin antibody [HL1229] used in Western Blot (WB). GTX636577
Anti-E-Cadherin antibody [HL1229] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636577
Anti-E-Cadherin antibody [HL1229] used in Western Blot (WB). GTX636577
Anti-E-Cadherin antibody [HL1229] used in IHC (Paraffin sections) (IHC-P). GTX636577
Anti-E-Cadherin antibody [HL1229] used in IHC (Paraffin sections) (IHC-P). GTX636577
Anti-E-Cadherin antibody [HL1229] used in IHC (Paraffin sections) (IHC-P). GTX636577
Anti-E-Cadherin antibody [HL1229] used in IHC (Paraffin sections) (IHC-P). GTX636577
Anti-E-Cadherin antibody [HL1229] used in Western Blot (WB). GTX636577
Anti-E-Cadherin antibody [HL1229] used in IHC (Paraffin sections) (IHC-P). GTX636577
Anti-E-Cadherin antibody [HL1229] used in Western Blot (WB). GTX636577
Anti-E-Cadherin antibody [HL1229] used in IHC (Paraffin sections) (IHC-P). GTX636577

目录号 GTX636577

文献引用 1

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL1229

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse, Rat, Cat, Dog
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

97 kDa. ( Note )

预测种属反应

Bovine, Pig(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human E-Cadherin. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

RRID

AB_2910011

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

cadherin 1 , Arc-1 , BCDS1 , CD324 , CDHE , ECAD , LCAM , UVO

细胞定位

Cell junction,Cell membrane,Endosome

功能与背景

This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. [provided by RefSeq, Nov 2015]

数据库

研究领域

產品图片

Anti-E-Cadherin antibody [HL1229] used in Western Blot (WB). GTX636577

GTX636577 WB Image

KO/KD

Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [HL1229] (GTX636577) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-E-Cadherin antibody [HL1229] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636577

GTX636577 ICC/IF Image

E-Cadherin antibody [HL1229] detects E-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: E-Cadherin stained by E-Cadherin antibody [HL1229] (GTX636577) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-E-Cadherin antibody [HL1229] used in Western Blot (WB). GTX636577

GTX636577 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [HL1229] (GTX636577) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-E-Cadherin antibody [HL1229] used in IHC (Paraffin sections) (IHC-P). GTX636577

GTX636577 IHC-P Image

E-Cadherin antibody [HL1229] detects E-Cadherin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
E-Cadherin stained by E-Cadherin antibody [HL1229] (GTX636577) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-E-Cadherin antibody [HL1229] used in IHC (Paraffin sections) (IHC-P). GTX636577

GTX636577 IHC-P Image

E-Cadherin antibody [HL1229] detects E-Cadherin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
E-Cadherin stained by E-Cadherin antibody [HL1229] (GTX636577) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-E-Cadherin antibody [HL1229] used in IHC (Paraffin sections) (IHC-P). GTX636577

GTX636577 IHC-P Image

E-Cadherin antibody [HL1229] detects E-Cadherin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
E-Cadherin stained by E-Cadherin antibody [HL1229] (GTX636577) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-E-Cadherin antibody [HL1229] used in IHC (Paraffin sections) (IHC-P). GTX636577

GTX636577 IHC-P Image

E-Cadherin antibody [HL1229] detects E-Cadherin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
E-Cadherin stained by E-Cadherin antibody [HL1229] (GTX636577) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-E-Cadherin antibody [HL1229] used in Western Blot (WB). GTX636577

GTX636577 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [HL1229] (GTX636577) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-E-Cadherin antibody [HL1229] used in IHC (Paraffin sections) (IHC-P). GTX636577

GTX636577 IHC-P Image

E-Cadherin antibody [HL1229] detects E-Cadherin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog mammary gland.
E-Cadherin stained by E-Cadherin antibody [HL1229] (GTX636577) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-E-Cadherin antibody [HL1229] used in Western Blot (WB). GTX636577

GTX636577 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [HL1229] (GTX636577) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-E-Cadherin antibody [HL1229] used in IHC (Paraffin sections) (IHC-P). GTX636577

GTX636577 IHC-P Image

E-Cadherin antibody [HL1229] detects E-Cadherin protein by immunohistochemical analysis.
Sample: Paraffin-embedded cat pancreas.
E-Cadherin stained by E-Cadherin antibody [HL1229] (GTX636577) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

文献引用

Application Reference

Pai JT et al. Int J Mol Sci 2022; 23 (3) Propolin G-Suppressed Epithelial-to-Mesenchymal Transition in Triple-Negative Breast Cancer Cells via Glycogen Synthase Kinase 3β-Mediated Snail and HDAC6-Regulated Vimentin Degradation.
实验应用 : WB
种属反应 : Human
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