Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [HL1229] (GTX636577) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [HL1229] (GTX636577) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

E-Cadherin antibody [HL1229] detects E-Cadherin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
E-Cadherin stained by E-Cadherin antibody [HL1229] (GTX636577) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

E-Cadherin antibody [HL1229] detects E-Cadherin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
E-Cadherin stained by E-Cadherin antibody [HL1229] (GTX636577) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

E-Cadherin antibody [HL1229] detects E-Cadherin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
E-Cadherin stained by E-Cadherin antibody [HL1229] (GTX636577) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

E-Cadherin antibody [HL1229] detects E-Cadherin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
E-Cadherin stained by E-Cadherin antibody [HL1229] (GTX636577) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [HL1229] (GTX636577) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

E-Cadherin antibody [HL1229] detects E-Cadherin protein by immunohistochemical analysis.
Sample: Paraffin-embedded cat pancreas.
E-Cadherin stained by E-Cadherin antibody [HL1229] (GTX636577) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

E-Cadherin antibody [HL1229] detects E-Cadherin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog mammary gland.
E-Cadherin stained by E-Cadherin antibody [HL1229] (GTX636577) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [HL1229] (GTX636577) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

E-Cadherin antibody [HL1229] detects E-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: E-Cadherin stained by E-Cadherin antibody [HL1229] (GTX636577) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).