产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
mouse brain , rat brain
预测种属反应
Human, Cat, Dog, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.77 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Mouse
免疫原
Recombinant protein encompassing a sequence within the N-terminus region of mouse EAAT2. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2887196
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
solute carrier family 1 (glial high affinity glutamate transporter), member 2 , 1700091C19Rik , 2900019G14Rik , AI159670 , Eaat2 , GLT-1 , GLT1 , MGLT1
细胞定位
Membrane
功能与背景
Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
数据库
研究领域
產品图片
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GTX134062 IHC-P Image
EAAT2 antibody detects EAAT2 protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded mouse hippocampus.EAAT2 stained by EAAT2 antibody (GTX134062) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX134062 WB Image
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EAAT2 antibody (GTX134062) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX134062 IHC-P Image
EAAT2 antibody detects EAAT2 protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded mouse eye.Green: EAAT2 stained by EAAT2 antibody (GTX134062) diluted at 1:250.Red: beta Tubulin 3/ Tuj1, a cytoplasm marker, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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