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EFHD2抗体

Anti-EFHD2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX108080
Anti-EFHD2 antibody used in Western Blot (WB). GTX108080
Anti-EFHD2 antibody used in Western Blot (WB). GTX108080
Anti-EFHD2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108080
Anti-EFHD2 antibody used in Western Blot (WB). GTX108080

目录号 GTX108080

宿主

Rabbit

克隆

Polyclonal

同种型

IgG

实验应用

WB, ICC/IF, IHC-Fr

种属反应

Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

27 kDa. ( Note )

阳性对照

H1299 , mouse brain , rat brain , EFHD2-transfected 293T

预测种属反应

Zebrafish, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

0.1M Tris, 0.1M Glycine, 10% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human EFHD2. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1950173

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

EF-hand domain family member D2 , SWS1

功能与背景

May regulate B-cell receptor (BCR)-induced immature and primary B-cell apoptosis (By similarity). Plays a role as negative regulator of the canonical NF-kappa-B-activating branch (By similarity). Controls spontaneous apoptosis through the regulation of BCL2L1 abundance.

数据库

研究领域

產品图片

Anti-EFHD2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX108080

GTX108080 IHC-Fr Image

EFHD2 antibody detects EFHD2 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: EFHD2 protein stained by EFHD2 antibody (GTX108080) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-EFHD2 antibody used in Western Blot (WB). GTX108080

GTX108080 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EFHD2 antibody (GTX108080) diluted at 1:1000.

Anti-EFHD2 antibody used in Western Blot (WB). GTX108080

GTX108080 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EFHD2 antibody (GTX108080) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EFHD2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108080

GTX108080 ICC/IF Image

EFHD2 antibody detects EFHD2 protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EFHD2 protein stained by EFHD2 antibody (GTX108080) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-EFHD2 antibody used in Western Blot (WB). GTX108080

GTX108080 WB Image

Sample(30 μg of whole cell lysate)
A:H1299
10% SDS PAGE
GTX108080 diluted at 1:1000

文献引用

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SDS
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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