产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
2 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Carrier-protein conjugated synthetic peptide surrounding mutant L858R of human EGFR. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
RRID
AB_2909906
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
epidermal growth factor receptor , ERBB , ERBB1 , HER1 , NISBD2 , PIG61 , mENA
细胞定位
Cell membrane,Endoplasmic reticulum membrane,Endosome,Endosome membrane,Nucleus
功能与背景
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016]
数据库
研究领域
產品图片
![Anti-EGFR (L858R Mutant) antibody [HL1022] used in Western Blot (WB). GTX635834](/templates/default/js/lightbox/images/loading.gif "Anti-EGFR (L858R Mutant) antibody [HL1022] used in Western Blot (WB). GTX635834")
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GTX635834 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR (L858R Mutant) antibody [HL1022] (GTX635834) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635834 WB Image
Various whole cell extracts (30 | 15 | 15 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR (L858R Mutant) antibody [HL1022] (GTX635834) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
PC-9 cells express EGFR (Glu746-Ala750 deletion) protein and H1299 cells express wide type EGFR protein. (refer to PMID: 22157369)
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文献引用
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产品评论
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![Anti-EGFR (L858R Mutant) antibody [HL1022] used in Western Blot (WB). GTX635834](/upload/website/prouct_img/small/GTX635834/GTX635834_44165_20220812_WB_B_22081423_256.webp "Anti-EGFR (L858R Mutant) antibody [HL1022] used in Western Blot (WB). GTX635834")
![Anti-EGFR (L858R Mutant) antibody [HL1022] used in Western Blot (WB). GTX635834](/upload/website/prouct_img/small/GTX635834/GTX635834_44165_20230217_WB_23022022_211.webp "Anti-EGFR (L858R Mutant) antibody [HL1022] used in Western Blot (WB). GTX635834")
![Anti-EGFR (L858R Mutant) antibody [HL1022] used in Western Blot (WB). GTX635834](/upload/website/prouct_img/normal/GTX635834/GTX635834_44165_20220812_WB_B_22081423_256.webp "Anti-EGFR (L858R Mutant) antibody [HL1022] used in Western Blot (WB). GTX635834")
![Anti-EGFR (L858R Mutant) antibody [HL1022] used in Western Blot (WB). GTX635834](/upload/website/prouct_img/normal/GTX635834/GTX635834_44165_20230217_WB_23022022_211.webp "Anti-EGFR (L858R Mutant) antibody [HL1022] used in Western Blot (WB). GTX635834")
