Various whole cell extracts (30 | 15 | 15 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR (L858R Mutant) antibody [HL1022] (GTX635834) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR (L858R Mutant) antibody [HL1022] (GTX635834) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.