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EGFR抗体 [GT461]

KO/KD Orthogonal IP/MS
Anti-EGFR antibody [GT461] used in Western Blot (WB). GTX628888
Anti-EGFR antibody [GT461] used in Western Blot (WB). GTX628888
Anti-EGFR antibody [GT461] used in Immunoprecipitation (IP). GTX628888
Anti-EGFR antibody [GT461] used in Western Blot (WB). GTX628888
Anti-EGFR antibody [GT461] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628888
Anti-EGFR antibody [GT461] used in Western Blot (WB). GTX628888

目录号 GTX628888

文献引用 4

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 GT461

同种型

 IgG2a

实验应用

 WB, ICC/IF, IHC-P, IP

种属反应

 Human
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 EGFR 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IP 1:100-1:500
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

134 kDa. ( Note )

Observed MW

180 kDa.

阳性对照

A431 , A549

产品说明

This antibody was raised against human EGFR Intracellular domain.
IP/MS validation was supported by references (PMID:30377424)

预测种属反应

Mouse, Rat, Pig(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.01 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the Intracellular domain of human EGFR. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein G.

偶联

Unconjugated

RRID

AB_2888065

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

epidermal growth factor receptor , ERBB , ERBB1 , HER1 , NISBD2 , PIG61 , mENA

细胞定位

Cell membrane; Single-pass type I membrane protein , Isoform 2: Secreted

功能与背景

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-EGFR antibody [GT461] used in Western Blot (WB). GTX628888

GTX628888 WB Image

KO/KD

Non-transfected (–) and transfected (+) A431 whole cell extracts (15 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [GT461] (GTX628888) diluted at 1:8000.

Anti-EGFR antibody [GT461] used in Western Blot (WB). GTX628888

GTX628888 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [GT461] (GTX628888) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-EGFR antibody [GT461] used in Immunoprecipitation (IP). GTX628888

GTX628888 IP Image

Immunoprecipitation of EGFR protein from A431 whole cell extracts using 5 μg of EGFR antibody [GT461] (GTX628888).
Western blot analysis was performed using EGFR antibody [GT461] (GTX628888).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-EGFR antibody [GT461] used in Western Blot (WB). GTX628888

GTX628888 WB Image

EGFR antibody [GT461] detects EGFR protein by Western blot analysis.
A. 30 μg A431 whole cell lysate/extract
5 % SDS-PAGE
EGFR antibody [GT461] (GTX628888) dilution: 1:10000

Anti-EGFR antibody [GT461] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628888

GTX628888 ICC/IF Image

EGFR antibody [GT461] detects EGFR protein at cell membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde for 10 min.
Green: EGFR protein stained by EGFR antibody [GT461] (GTX628888) diluted at 1:300.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-EGFR antibody [GT461] used in Western Blot (WB). GTX628888

GTX628888 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [GT461] (GTX628888) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

文献引用

Application Reference

Zih-Yin Lai et al. Int J Mol Sci 2021; 22 (16) Gain-of-Function Mutant TP53 R248Q Overexpressed in Epithelial Ovarian Carcinoma Alters AKT-Dependent Regulation of Intercellular Trafficking in Responses to EGFR/MDM2 Inhibitor.
实验应用 : ICC/IF
种属反应 : Human
Lai Z.Y. et al. Int. J. Mol. Sci. 2021; 22 (16) : 8784 Gain-of-Function Mutant TP53 R248Q Overexpressed in Epithelial Ovarian Carcinoma Alters AKT-Dependent Regulation of Intercellular Trafficking in Responses to EGFR/MDM2 Inhibitor.
实验应用 : WB, ICC/IF
种属反应 : Human
Boyle J et al. Endocrine 2019; (Epub) CRH stimulation improves 18F-FDG-PET detection of pituitary adenomas in Cushing's disease.
实验应用 : IHC-P
种属反应 : Human
Sikorski K et al. Nat Methods 2018; 15 (11) : 909-912 A high-throughput pipeline for validation of antibodies.
实验应用 : IP
种属反应 : Human
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产品包装 报价 (¥)
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¥ 1700
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