产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
Observed MW
65 kDa.
阳性对照
A431 , A549 , H1299 , HCT116
预测种属反应
Mouse, Rat, Pig(>80% identity)
产品属性
形式
Liquid
存储溶液
0.1M Tris, 0.1M Glycine, 10% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Synthetic peptide encompassing a sequence within the Extracellular domain of human EGFR. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_1950177
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
epidermal growth factor receptor , ERBB , ERBB1 , HER1 , NISBD2 , PIG61 , mENA
细胞定位
Cell membrane; Single-pass type I membrane protein , Isoform 2: Secreted
功能与背景
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq]
数据库
研究领域
產品图片
![Anti-EGFR antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX100725](/templates/default/js/lightbox/images/loading.gif "Anti-EGFR antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX100725")
|
GTX100725 IHC-P Image
Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using EGFR(GTX100725) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
|
|
|
GTX100725 WB Image
Non-transfected (–) and transfected (+) A431 whole cell extracts (15 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [N1], N-term (GTX100725) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX100725 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [N1], N-term (GTX100725) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
|
|
|
GTX100725 WB Image
EGFR antibody detects EGFR protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody (GTX100725) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX100725 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR antibody [N1], N-term (GTX100725) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX100725 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human breast cancer, using EGFR(GTX100725) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
|
文献引用
目前没有使用 EGFR antibody [N1], N-term (GTX100725) 的文献被收录。快来回报我们第一篇参考文献吧！
产品评论
目前没有使用 EGFR antibody [N1], N-term (GTX100725) 的产品评论被收录。快发表第一篇产品评论吧！
![Anti-EGFR antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX100725](/upload/website/prouct_img/small/GTX100725/GTX100725_IHC_w_23060100_379.webp "Anti-EGFR antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX100725")
![Anti-EGFR antibody [N1], N-term used in Western Blot (WB). GTX100725](/upload/website/prouct_img/small/GTX100725/GTX100725_39995_20160630_WB_shRNA_watermark_w_23060100_655.webp "Anti-EGFR antibody [N1], N-term used in Western Blot (WB). GTX100725")
![Anti-EGFR antibody [N1], N-term used in Western Blot (WB). GTX100725](/upload/website/prouct_img/small/GTX100725/GTX100725_39995_20200131_WB_TPM_watermark_w_23060100_603.webp "Anti-EGFR antibody [N1], N-term used in Western Blot (WB). GTX100725")
![Anti-EGFR antibody [N1], N-term used in Western Blot (WB). GTX100725](/upload/website/prouct_img/small/GTX100725/GTX100725_39995_20141231_WB_w_23060100_761.webp "Anti-EGFR antibody [N1], N-term used in Western Blot (WB). GTX100725")
![Anti-EGFR antibody [N1], N-term used in Western Blot (WB). GTX100725](/upload/website/prouct_img/small/GTX100725/GTX100725_39995_20180601_WB_w_23060100_916.webp "Anti-EGFR antibody [N1], N-term used in Western Blot (WB). GTX100725")
![Anti-EGFR antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX100725](/upload/website/prouct_img/small/GTX100725/GTX100725_39995_IHC2_w_23060100_158.webp "Anti-EGFR antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX100725")
![Anti-EGFR antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX100725](/upload/website/prouct_img/normal/GTX100725/GTX100725_IHC_w_23060100_379.webp "Anti-EGFR antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX100725")
![Anti-EGFR antibody [N1], N-term used in Western Blot (WB). GTX100725](/upload/website/prouct_img/normal/GTX100725/GTX100725_39995_20160630_WB_shRNA_watermark_w_23060100_655.webp "Anti-EGFR antibody [N1], N-term used in Western Blot (WB). GTX100725")
![Anti-EGFR antibody [N1], N-term used in Western Blot (WB). GTX100725](/upload/website/prouct_img/normal/GTX100725/GTX100725_39995_20200131_WB_TPM_watermark_w_23060100_603.webp "Anti-EGFR antibody [N1], N-term used in Western Blot (WB). GTX100725")
![Anti-EGFR antibody [N1], N-term used in Western Blot (WB). GTX100725](/upload/website/prouct_img/normal/GTX100725/GTX100725_39995_20141231_WB_w_23060100_761.webp "Anti-EGFR antibody [N1], N-term used in Western Blot (WB). GTX100725")
![Anti-EGFR antibody [N1], N-term used in Western Blot (WB). GTX100725](/upload/website/prouct_img/normal/GTX100725/GTX100725_39995_20180601_WB_w_23060100_916.webp "Anti-EGFR antibody [N1], N-term used in Western Blot (WB). GTX100725")
![Anti-EGFR antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX100725](/upload/website/prouct_img/normal/GTX100725/GTX100725_39995_IHC2_w_23060100_158.webp "Anti-EGFR antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX100725")
