产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
A431 (Starvation for 16 hr and then 100 ng/ml EGF treatment for 5 min)
产品说明
The full-length EGFR includes the 24 aa signal peptide that is cleaved during EGFR maturation. The Tyr1086 of mature EGFR is equivalent to Tyr1110 of uncleaved EGFR.
预测种属反应
Mouse, Rat, Pig(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 1% BSA, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.11 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Carrier-protein conjugated synthetic peptide surrounding phospho Tyr1086 of human EGFR. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2887041
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
epidermal growth factor receptor , ERBB , ERBB1 , HER1 , NISBD2 , PIG61 , mENA
细胞定位
Cell membrane; Single-pass type I membrane protein , Isoform 2: Secreted
功能与背景
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX133599 WB Image
Untreated (–) and treated (+) A431 whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr1086) antibody (GTX133599) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX133599 ICC/IF Image
EGFR (phospho Tyr1086) antibody detects EGFR (phospho Tyr1086) protein at cell membrane by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EGFR (phospho Tyr1086) protein stained by EGFR (phospho Tyr1086) antibody (GTX133599) diluted at 1:500. Blue: Hoechst 33342 staining.
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GTX133599 IHC-P Image
EGFR (phospho Tyr1086) antibody detects EGFR (phospho Tyr1086) protein at cell membrane in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. EGFR (phospho Tyr1086) antibody (GTX133599) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献引用
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产品评论
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