产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
EGF treated A431 cell
产品说明
The full-length EGFR includes the 24 aa signal peptide that is cleaved during EGFR maturation. The Tyr845 of mature EGFR is equivalent to Tyr869 of uncleaved EGFR.
预测种属反应
Mouse, Rat, Chicken, Pig(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 1% BSA, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.6 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Carrier-protein conjugated synthetic peptide surrounding phospho Tyr845 of human EGFR. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2887042
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
epidermal growth factor receptor , ERBB , ERBB1 , HER1 , NISBD2 , PIG61 , mENA
细胞定位
Cell membrane; Single-pass type I membrane protein , Isoform 2: Secreted
功能与背景
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq]
数据库
研究领域
產品图片
|
GTX133600 ICC/IF Image
EGFR (phospho Tyr845) antibody detects EGFR (phospho Tyr845) protein at cell membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EGFR (phospho Tyr845) protein stained by EGFR (phospho Tyr845) antibody (GTX133600) diluted at 1:500.
Blue: Hoechst 33342 staining.
|
|
|
GTX133600 IHC-P Image
EGFR (phospho Tyr845) antibody detects EGFR (phospho Tyr845) protein at cell membrane in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
EGFR (phospho Tyr845) antibody (GTX133600) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX133600 WB Image
Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr845) antibody (GTX133600) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
文献引用
产品评论
目前没有使用 EGFR (phospho Tyr845) antibody (GTX133600) 的产品评论被收录。快发表第一篇产品评论吧！
