产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:10000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
PC-12 , Rat2
预测种属反应
Zebrafish, Sheep, Bovine, Xenopus tropicalis(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 1% BSA, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.4 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Full length human EIF5A Recombinant protein.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_1950191
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
eukaryotic translation initiation factor 5A , EIF-5A , EIF5A1 , eIF5AI
细胞定位
Cytoplasm , Nucleus , Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side , nuclear pore complex
功能与背景
mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of TP53/p53 and TP53/p53-dependent apoptosis. Regulates also TNF-alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts.
数据库
研究领域
產品图片
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GTX111013 WB Image
EIF5A antibody detects EIF5A protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
12 % SDS-PAGE
EIF5A antibody (GTX111013) dilution: 1:5000
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GTX111013 WB Image
Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with EIF5A antibody (GTX111013) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX111013 ICC/IF Image
EIF5A antibody detects EIF5A protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: EIF5A stained by EIF5A antibody (GTX111013) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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GTX111013 IHC-P Image
EIF5A antibody detects EIF5A protein at cytoplasm in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
EIF5A antibody (GTX111013) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX111013 WB Image
Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with EIF5A antibody (GTX111013) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献引用
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