产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:1000-1:10000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
预测种属反应
Zebrafish, Sheep, Drosophila, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1.2 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human EIF6. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_11173506
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
eukaryotic translation initiation factor 6 , CAB , EIF3A , ITGB4BP , b(2)gcn , eIF-6 , p27(BBP) , p27BBP
细胞定位
Cytoplasm , Nucleus
功能与背景
Hemidesmosomes are structures which link the basal lamina to the intermediate filament cytoskeleton. An important functional component of hemidesmosomes is the integrin beta-4 subunit (ITGB4), a protein containing two fibronectin type III domains. The protein encoded by this gene binds to the fibronectin type III domains of ITGB4 and may help link ITGB4 to the intermediate filament cytoskeleton. The encoded protein, which is insoluble and found both in the nucleus and in the cytoplasm, can function as a translation initiation factor and prevent the association of the 40S and 60S ribosomal subunits. Multiple transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]
数据库
研究领域
產品图片
![Anti-EIF6 antibody [N1C3-2] used in Western Blot (WB). GTX117971](/templates/default/js/lightbox/images/loading.gif "Anti-EIF6 antibody [N1C3-2] used in Western Blot (WB). GTX117971")
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GTX117971 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EIF6 antibody [N1C3-2] (GTX117971) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX117971 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EIF6 antibody [N1C3-2] (GTX117971) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX117971 IHC-P Image
EIF6 antibody [N1C3-2] detects EIF6 protein at cytoplasm and nucleus in human colon by immunohistochemical analysis.
Sample: Paraffin-embedded human colon.
EIF6 antibody [N1C3-2] (GTX117971) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX117971 WB Image
Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EIF6 antibody [N1C3-2] (GTX117971) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX117971 WB Image
Various whole cell extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EIF6 antibody [N1C3-2] (GTX117971) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献引用
产品评论
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![Anti-EIF6 antibody [N1C3-2] used in Western Blot (WB). GTX117971](/upload/website/prouct_img/small/GTX117971/GTX117971_43649_20221118_WB_shRNA_watermark_22112219_941.webp "Anti-EIF6 antibody [N1C3-2] used in Western Blot (WB). GTX117971")
![Anti-EIF6 antibody [N1C3-2] used in Western Blot (WB). GTX117971](/upload/website/prouct_img/small/GTX117971/GTX117971_43649_20190726_WB_w_23060519_481.webp "Anti-EIF6 antibody [N1C3-2] used in Western Blot (WB). GTX117971")
![Anti-EIF6 antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX117971](/upload/website/prouct_img/small/GTX117971/GTX117971_40492_20160518_IHC-P_H_w_23060519_420.webp "Anti-EIF6 antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX117971")
![Anti-EIF6 antibody [N1C3-2] used in Western Blot (WB). GTX117971](/upload/website/prouct_img/small/GTX117971/GTX117971_43649_20220923_WB_M_R_22092622_364.webp "Anti-EIF6 antibody [N1C3-2] used in Western Blot (WB). GTX117971")
![Anti-EIF6 antibody [N1C3-2] used in Western Blot (WB). GTX117971](/upload/website/prouct_img/small/GTX117971/GTX117971_43649_20221007_WB_M_R_22101319_898.webp "Anti-EIF6 antibody [N1C3-2] used in Western Blot (WB). GTX117971")
![Anti-EIF6 antibody [N1C3-2] used in Western Blot (WB). GTX117971](/upload/website/prouct_img/normal/GTX117971/GTX117971_43649_20221118_WB_shRNA_watermark_22112219_941.webp "Anti-EIF6 antibody [N1C3-2] used in Western Blot (WB). GTX117971")
![Anti-EIF6 antibody [N1C3-2] used in Western Blot (WB). GTX117971](/upload/website/prouct_img/normal/GTX117971/GTX117971_43649_20190726_WB_w_23060519_481.webp "Anti-EIF6 antibody [N1C3-2] used in Western Blot (WB). GTX117971")
![Anti-EIF6 antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX117971](/upload/website/prouct_img/normal/GTX117971/GTX117971_40492_20160518_IHC-P_H_w_23060519_420.webp "Anti-EIF6 antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX117971")
![Anti-EIF6 antibody [N1C3-2] used in Western Blot (WB). GTX117971](/upload/website/prouct_img/normal/GTX117971/GTX117971_43649_20220923_WB_M_R_22092622_364.webp "Anti-EIF6 antibody [N1C3-2] used in Western Blot (WB). GTX117971")
![Anti-EIF6 antibody [N1C3-2] used in Western Blot (WB). GTX117971](/upload/website/prouct_img/normal/GTX117971/GTX117971_43649_20221007_WB_M_R_22101319_898.webp "Anti-EIF6 antibody [N1C3-2] used in Western Blot (WB). GTX117971")
