产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500 - 1:2000 |
| 1:50 - 1:200 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS, 50% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
Batch dependent (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human EIF6 (NP_852133.1).
纯化方式
Purified by affinity chromatography
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
eukaryotic translation initiation factor 6 , CAB , EIF3A , ITGB4BP , b(2)gcn , eIF-6 , p27(BBP) , p27BBP
细胞定位
Cytoplasm,Nucleolus
功能与背景
Hemidesmosomes are structures which link the basal lamina to the intermediate filament cytoskeleton. An important functional component of hemidesmosomes is the integrin beta-4 subunit (ITGB4), a protein containing two fibronectin type III domains. The protein encoded by this gene binds to the fibronectin type III domains of ITGB4 and may help link ITGB4 to the intermediate filament cytoskeleton. The encoded protein, which is insoluble and found both in the nucleus and in the cytoplasm, can function as a translation initiation factor and prevent the association of the 40S and 60S ribosomal subunits. Multiple non-protein coding transcript variants and variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2012]
数据库
研究领域
產品图片
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GTX54010 ICC/IF Image
ICC/IF analysis of U2OS cells using GTX54010 EIF6 antibody.
Blue : DAPI
Dilution : 1:50
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GTX54010 WB Image
WB analysis of various sample lysates using GTX54010 EIF6 antibody.
Dilution : 1:1000
Loading : 25μg per lane
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GTX54010 ICC/IF Image
ICC/IF analysis of MCF7 cells using GTX54010 EIF6 antibody.
Blue : DAPI
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GTX54010 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX54010 EIF6 antibody.
Blue : DAPI
Dilution : 1:50
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