ENO1抗体 [N3C3]

Name: ENO1抗体 [N3C3]
Brand: GeneTex
SKU: GTX101803
Price: 4000.00 RMB

Anti-ENO1 antibody [N3C3] used in Western Blot (WB). GTX101803

Anti-ENO1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101803

Anti-ENO1 antibody [N3C3] used in IHC (Whole mount) (IHC-Wm). GTX101803

Anti-ENO1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101803

目录号 GTX101803

文献引用 6

宿主	Rabbit
克隆	Polyclonal
同种型	IgG
实验应用	WB, ICC/IF, IHC-P, IHC-Wm, Dot
种属反应	Human, Mouse, Rat, Zebrafish
产品包装	100 μl (¥4000), 25 μl (¥1700)

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View product citations for antibody GTX101803 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:500-1:20000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Wm	Assay dependent
Dot	Assay dependent

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

47 kDa. ( Note )

阳性对照

Mouse brain , A431 , H1299 , 293T

预测种属反应

Bovine, Chicken, Guinea pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

0.1M Tris, 0.1M Glycine, 10% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human ENO1. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1950200

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

enolase 1 , ENO1L1 , HEL-S-17 , MPB1 , NNE , PPH

细胞定位

Cytoplasm , Cell membrane , myofibril , sarcomere , M-band , Isoform MBP-1: Nucleus

功能与背景

This gene encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome. [provided by RefSeq]

数据库

Gene ID: 2023 ENO1
UniProt: P06733 ENO1

研究领域

產品图片

GTX101803 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [N3C3] (GTX101803) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101803 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using ENO1 antibody [N3C3] (GTX101803) at 1:300 dilution.

GTX101803 IHC-Wm Image

ENO1 antibody [N3C3] detects Eno1 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
ENO1 antibody [N3C3] (GTX101803) dilution: 1:100.

GTX101803 WB Image

Sample (30 μg of whole cell lysate)
A: zebrafish eye
10% SDS PAGE
GTX101803 diluted at 1:1000

GTX101803 ICC/IF Image

ENO1 antibody [N3C3] detects ENO1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ENO1 protein stained by ENO1 antibody [N3C3] (GTX101803) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

GTX101803 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX101803 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101803 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [N3C3] (GTX101803) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX101803 WB Image

Sample (30 μg of whole cell lysate)
A: A431 (GTX27909)
B: H1299
10% SDS PAGE
GTX101803 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101803 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric tissue, using ENO1(GTX101803) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101803 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [N3C3] (GTX101803) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101803 WB Image

The data was published in the journal BMC Genomics in 2015. PMID: 25652794

文献引用

Application Reference

Elisa De Tomi et al. Int J Mol Sci 2022; 23 (3) Upregulation of miR-34a-5p, miR-20a-3p and miR-29a-3p by Onconase in A375 Melanoma Cells Correlates with the Downregulation of Specific Onco-Proteins.

实验应用 : WB

种属反应 : Human

Chang SJ et al. Arch Biochem Biophys 2018; 647 : 10-32 Proteomic investigating the cooperative lethal effect of EGFR and MDM2 inhibitors on ovarian carcinoma.

实验应用 : WB

种属反应 : Human

Zhou X et al. Technol Cancer Res Treat 2016; 15 (5) : 697-706 Identification of Differentiation-Related Proteins in Gastric Adenocarcinoma Tissues by Proteomics.

实验应用 : WB

种属反应 : Human

Lin LL et al. BMC Genomics 2015; 16 : 41 Integrating transcriptomics and proteomics to show that tanshinone IIA suppresses cell growth by blocking glucose metabolism in gastric cancer cells.

实验应用 : WB

种属反应 : Human

Wu YH et al. PLoS One 2013; 8 (8) : e70642 Quantitative proteomic analysis of human lung tumor xenografts treated with the ectopic ATP synthase inhibitor citreoviridin.

实验应用 : WB

种属反应 : Human

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Application Reference

Elisa De Tomi et al. Int J Mol Sci 2022; 23 (3) Upregulation of miR-34a-5p, miR-20a-3p and miR-29a-3p by Onconase in A375 Melanoma Cells Correlates with the Downregulation of Specific Onco-Proteins.

实验应用 : WB

种属反应 : Human

Chang SJ et al. Arch Biochem Biophys 2018; 647:10-32 Proteomic investigating the cooperative lethal effect of EGFR and MDM2 inhibitors on ovarian carcinoma.

实验应用 : WB

种属反应 : Human

Zhou X et al. Technol Cancer Res Treat 2016; 15 (5):697-706 Identification of Differentiation-Related Proteins in Gastric Adenocarcinoma Tissues by Proteomics.

实验应用 : WB

种属反应 : Human

Lin LL et al. BMC Genomics 2015; 16:41 Integrating transcriptomics and proteomics to show that tanshinone IIA suppresses cell growth by blocking glucose metabolism in gastric cancer cells.

实验应用 : WB

种属反应 : Human

Wu YH et al. PLoS One 2013; 8 (8):e70642 Quantitative proteomic analysis of human lung tumor xenografts treated with the ectopic ATP synthase inhibitor citreoviridin.

实验应用 : WB

种属反应 : Human

Hsiao KC et al. PLoS One 2013; 8 (7):e69354 Surface α-enolase promotes extracellular matrix degradation and tumor metastasis and represents a new therapeutic target.

实验应用 : Dot

SDS
Glycerol.pdf
Thimerosal.pdf

产品包装	报价 (¥)
100 μl	¥ 4000
25 μl	¥ 1700
Qty

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	http://www.neobioscience.com
	genetex@neobioscience.com

ENO1抗体 [N3C3]

目录号 GTX101803

宿主

克隆

同种型

实验应用

种属反应

产品应用

应用说明

预期分子量

阳性对照

预测种属反应

产品属性

形式

存储溶液

存放说明

浓度

免疫原种属

免疫原

纯化方式

偶联

RRID

注意事项

相关讯息

别名

细胞定位

功能与背景

数据库

研究领域

產品图片

GTX101803 WB Image

GTX101803 IHC-P Image

GTX101803 IHC-Wm Image

GTX101803 WB Image

GTX101803 ICC/IF Image

GTX101803 WB Image

GTX101803 WB Image

GTX101803 WB Image

GTX101803 IHC-P Image

GTX101803 WB Image

GTX101803 WB Image

文献引用

Application Reference

产品评论

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