产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
A431 nuclear extract
预测种属反应
Chimpanzee, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1.29 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human EP300. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2887243
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
E1A binding protein p300 , KAT3B , MKHK2 , RSTS2 , p300
细胞定位
Cytoplasm,Nucleus
功能与背景
This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
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GTX134238 IHC-P Image
EP300 antibody detects EP300 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse stomach.EP300 stained by EP300 antibody (GTX134238) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX134238 WB Image
A431 whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with EP300 antibody (GTX134238) diluted at 1:500 and competitor's antibody (sc-584) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX134238 IHC-P Image
EP300 antibody detects EP300 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.EP300 stained by EP300 antibody (GTX134238) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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